Ancell

anti-CD244 [2B4] (human), mAb (ANC244.8/8F7) (preservative free)

CHF 306.00

In stock

ANC-239-820100 µgCHF 306.00

Figure 1: Binding of anti-CD244 (ANC244.8/8F7) +GAM/FITC to human PBL. Method: Five x 10^5 ficoll prepared human PBMC were washed and incubated 45 minutes on ice with 80ul of anti-CD244 (clone ANC244.8) antibody at 10ug/ml. Cells were washed twice and incubated with 2nd reagent Goat anti-Mouse IgG/FITC (#ANC-232-011), after which they were washed three times, fixed and analyzed by FACS using a lymphocyte gate. A net 43% sub population of cells stained positive with a mean shift of 0.83 log10 fluorescent units when compared to a Mouse IgG1 negative control (#ANC-278-010).

Specifications / Handling

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Product Details
Synonyms	Natural Killer Cell Receptor 2B4; NK Cell Activation-inducing Ligand; NAIL; NKR2B4; SLAMF4
Product Type	Monoclonal Antibody
Properties
Clone	ANC244.8/8F7
Isotype	Mouse IgG1κ
Source/Host	Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen	Recombinant soluble human CD244.
Label/Conjugates	Preservative Free
Application	ELISA Flow Cytometry
Crossreactivity	Human
Specificity	Antibody ANC244.8 binds to recombinant CD244-muIg (#ANC-544-020) in EIA, and to cell surface CD244 on human PBL in FACS. ANC244.8 binds to a CD244 epitope that is distinct from the binding sites of clone ANC2B4/3B12 (#ANC-259-020). It blocks binding of clone C1.7.
Purity	≥95% (SDS-PAGE)
Purity Detail	Protein A purified.
Concentration	1.0mg/ml
Formulation	50mM Sodium Phosphate pH 7.5, 100mM KCl, 150mM NaCl.
Isotype Negative Control	Mouse IgG1 Isotype Control
Other Product Data	Click here for Original Manufacturer Product Datasheet Our product description may differ slightly from the original manufacturers product datasheet.
Accession Number	Q9BZW8
Declaration	Manufactured by Ancell Corporation.
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	+4°C
Handling Advice	Avoid freeze/thaw cycles. Keep sterile.
Use/Stability	Stable for at least 1 year after receipt when stored at +4°C.
Documents
MSDS	Inquire
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

CD244 (2B4; SLAMF4) is a 66kDa member of the CD2/SLAM related receptor family (SRR). It is expressed on NK, T cell subsets, monocytes and eosinophils. It is expressed at high levels on leuikemia initiating cells, and plays a role in their proliferative potential. In mice there are two transcription variants, a long form and a short form of CD244 each of which have distinct signaling properties. In humans, only the long form is expressed. Decreased CD244 expression on monocytes of SLE patients correlated (negatively) with autoantibody titer. CD244 mediated function in immune response can vary greatly dependant on the context of its ligation.

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