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Chemodex
5-CFDA
71
CHF
CHF 71.00
In stock
CDX-C0039-M02525 mgCHF 71.00
CDX-C0039-M100100 mgCHF 206.00
CDX-C0039-G0011 gCHF 1'373.00
| Product Details | |
|---|---|
| Synonyms | 5-Carboxyfluorescein diacetate |
| Product Type | Chemical |
| Properties | |
| Formula |
C25H16O9 |
| MW | 460.39 |
| CAS | 79955-27-4 |
| Source/Host Chemicals | Synthetic. |
| Purity Chemicals | ≥95% (HPLC) |
| Appearance | White to yellow solid. |
| Solubility | Soluble in DMSO. |
| Identity | Determined by 1H-NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | WPUZGNPQMIWOHE-UHFFFAOYSA-N |
| Smiles | CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)C(O)=O)C1=CC=C(OC(C)=O)C=C1O2 |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice | Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Carboxyfluorescein diacetate (CFDA), which was originally used to measure intracellular pH but was soon adapted for use as a cell viability indicator. Upon hydrolysis by intracellular nonspecific esterases, CFDA forms carboxyfluorescein. As compared with fluorescein, carboxyfluorescein contains extra negative charges and is therefore better retained in cells CFDA is moderately permeant to most cell membranes, with uptake greater at pH 6.2 than at pH 7.41. CFDA has been used as a viability probe with a variety of cells, including bacteria, fungi (e.g., Saccharomyces cerevisiae), spermatozoa, natural killer (NK) cells and tumor cells.
Product References
(1) J. Immun. Methods 172, 115 (1994) | (2) Anticancer Res. 14, 927 (1994) | (3) Appl. Environ Microbiol. 60, 1467 (1994) | (4) Cytometry 15, 213 (1994) | (5) Biol. Reprod. 34, 127 (1986) | (6) Immun. Lett. 2, 187 (1981)
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