Chemodex

DAF-4 DA

CHF 285.00

In stock

CDX-D0216-M0011 mgCHF 285.00

CDX-D0216-M0055 mgCHF 1'139.00

CDX-D0216-M01010 mgCHF 2'035.00

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Product Details
Synonyms	DAF-4 diacetate; 3,4-Diamino-2',7'-dichlorofluorescein diacetate
Product Type	Chemical
Properties
Formula	C₂₄H₁₆Cl₂N₂O₇
MW	515.30
CAS	208850-34-4 (parent compound DAF-4)
Source/Host Chemicals	Synthetic.
Purity Chemicals	≥90% (HPLC)
Appearance	Light yellow powder.
Solubility	Soluble in chloroform or DMSO.
Identity	Determined by ¹H-NMR.
Declaration	Manufactured by Chemodex.
Other Product Data	Click here for Original Manufacturer Product Datasheet Our product description may differ slightly from the original manufacturers product datasheet.
InChi Key	OOSGYDWTQGDKAY-UHFFFAOYSA-N
Smiles	ClC1=C(OC(C)=O)C=C(OC(C=C(OC(C)=O)C(Cl)=C2)=C2C34OC(C5=C4C=C(N)C(N)=C5)=O)C3=C1
Shipping and Handling
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use/Stability	Stable for at least 2 years after receipt when stored at -20°C.
Documents
MSDS	Inquire
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

DAF-4 is a indicator for nitric oxide. As with other fluorescein diacetates (DAFs), the non-fluorescent DAF-4 diacetate (DAF-4 DA) is membrane permeant and is deacetylated by intracellular esterases to form DAF-4, which than can ract with NO to form the fluorescent DAF-4T. Can be used for the fluorimetric detection of nitric oxide and in fluorescence microscopy to measure real-time changes in nitric oxide levels in vivo. Spectral Data: λex 505nm; λem 530nm.

Product References

(1) H. Kojima, et al.; Anal. Chem. 70, 2446 (1998) | (2) N. Nakatsubo, et al.; FEBS Lett. 427, 263 (1998) | (3) T. Nagano; Luminescence 14, 283 (1999) (Review) | (4) T. Nagano; Proc. Jpn. Acad. Ser. B 86, 837 (2010) (Review)

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