Fluo-3 AM

CHF 249.00
In stock
CDX-F0033-M0011 mgCHF 249.00
CDX-F0033-M01010 mgCHF 2'016.00
More Information
Product Details
Synonyms Fluo-3 AM
Product Type Chemical
Properties
Formula C51H50Cl2N2O23
MW 1129.85
CAS 121714-22-5
Source/Host Chemicals Synthetic.
Purity Chemicals ≥90% (TLC)
Appearance Dark red powder.
Solubility Soluble in DMSO.
Identity Determined by 1H-NMR.
Declaration Manufactured by Chemodex.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

InChi Key PTPUOMXKXCCSEN-UHFFFAOYSA-N
Smiles CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)C1=CC=C(C=C1OCCOC1=C(C=CC(C)=C1)N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)C1=C2C=C(Cl)C(=O)C=C2OC2=CC(OCOC(C)=O)=C(Cl)C=C12
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Ca2+-indicator fluo-3 for use with visible-light excitation sources in flow cytometry and confocal laser-scanning microscopy. Since about 1996, fluo-3 has also been extensively used in cell-based high-throughput screening assays for drug discovery. Fluo-3 AM is essentially nonfluorescent until it is hydrolyzed intracellularly and/or in the presence of Ca2+. The green-fluorescent emission (~525 nm) of Ca2+-bound fluo-3 is conventionally detected using optical filter sets designed for fluorescein (FITC). As prepared by Minta, Kao and Tsien, fluo-3 was originally reported to undergo an ~40-fold increase in fluorescence upon binding Ca2+.

Product References

(1) T. Toyofuku et al.; Geochem., Geophys., Geosyst. 9(5) | (2008) | (2) D.M. Plank et al.; Meth. Cell Sc. 25(3-4), 123 (2004) | (3) P. Vandenberghe et al.; J. Immun.Meth. 127, 197 (1990) | (4) J.P.Y. Kao et al.; J. Biol. Chem. 264, 8179 (1989)

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