AdipoGen Life Sciences

anti-Caspase-1 (p10) (mouse), mAb (Casper-2)

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AG-20B-0044-C100100 µgCHF 480.00

Figure 1: Mouse Caspase-1 (p10) is detected by immunoblotting using anti-Caspase-1 (p10) (mouse), mAb (Casper-2) (Prod. No AG-20B-0044). Method: Caspase-1 was analyzed by Western blot in supernatants of differentiated bone marrow-derived dendritic cells (BMDCs) from wild-type and caspase-1^-/- mice activated or not by 5 μM Nigericin for 30 min (Prod. No. AG-CN2-0020). Supernatants (30μl) were separated by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with anti-Caspase-1 (p10) (mouse), mAb (Casper-2) (1μg /ml). Proteins were visualized by a chemiluminescence detection system.

Specifications / Handling

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Product Details
Synonyms	Interleukin-1β Convertase; IL-1BC; Interleukin-1β-converting Enzyme; ICE
Product Type	Monoclonal Antibody
Properties
Clone	Casper-2
Isotype	Mouse IgG2a
Source/Host	Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen	Recombinant mouse caspase-1.
Application	Western Blot (see online protocol): (1μg/ml) (no need to precipitate the cell supernatant for the detection of caspase-1 (mouse) upon inflammasome activation)
Crossreactivity	Mouse
Specificity	Recognizes endogenous full-length and activated (p10 fragment) mouse caspase-1.
Purity	≥95% (SDS-PAGE)
Purity Detail	Protein G-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Isotype Negative Control	Mouse IgG2a Isotype Control
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Documents
Protocols	Download PDF
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Scientific Background Information

Product Description

Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1β (IL-1β) and IL-18 and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3 responds to a variety of agonists including crystals.

Product-specific References

Measuring the inflammasome: O. Gross; Methods Mol. Biol. 844, 199 (2012) [PMID: 22262445]
XIAP Restricts TNF- and RIP3-Dependent Cell Death and Inflammasome Activation: M. Yabal, et al.; Cell Rep. 7, 1796 (2014) [PMID: 24882010]
AMP-activated protein kinase suppresses urate crystal-induced inflammation and transduces colchicine effects in macrophages: Y. Wang, et al.; Ann. Rheum. Dis. 75, 286 (2016) [PMC4417082]
Bile acids control inflammation and metabolic disorder through inhibition of NLRP3 inflammasome: C. Guo, et al.; Immunity 45, 802 (2016) [PMID: 27692610]
IL-36 Signaling Facilitates Activation of the NLRP3 Inflammasome and IL-23/IL-17 Axis in Renal Inflammation and Fibrosis: H.-H. Chi, et al.; J. Am. Soc. Nephrol. 28, 2022 (2017) [PMC5491282]
Interleukin-18 Is Critical for Mucosa-Associated Invariant T Cell Gamma Interferon Responses to Francisella Species In Vitro but Not In Vivo: E. Jesteadt, et al.; Infect. Immun. 86, e00117 (2018) [PMC5913842]
Caspase-1 self-cleavage is an intrinsic mechanism to terminate inflammasome activity: D. Boucher, et al.; J. Exp. Med. 215, 827 (2018) [PMC5839769]
Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages: C. Guo, et al.; Immunity 49, 842 (2018) [PMID: 30366764]
Repositioning of the β-Blocker Carvedilol as a Novel Autophagy Inducer That Inhibits the NLRP3 Inflammasome: W.-T. Wong, et al.; Front. Immunol. 9, 1920 (2018) [PMC6113403]
HSP70 is a negative regulator of NLRP3 inflammasome activation: P. Martine, et al.; Cell Death Diff. 10, 256 (2019) [PMC6420651]
Inflammasome Assays In Vitro and in Mouse Models: H. Guo & J.P.-Y. Ting; Curr. Protoc. Immunol. 131, e107 (2020) [PMC7751762]
Critical Role for the NLRP3 Inflammasome in Mediating IL-1β Production in Shigella sonnei-Infected Macrophages: L.-H. Li, et al. Front. Immunol. 11, 1115 (2020) [PMC7283925]
Heat shock and HSP70 regulate 5-FU-mediated caspase-1 activation in myeloid-derived suppressor cells and tumor growth in mice: T. Pilot, et al.; J. Immunother. Cancer 8, e000478 (2020) [PMC7228666]
Cathepsin B Is Required for NLRP3 Inflammasome Activation in Macrophages, Through NLRP3 Interaction: A. Chevriaux, et al.; Sec. Mol. Cell. Pathol. 8, 167 (2020) [PMC7162607]
Mechanism of miR-126 in hypoxia-reoxygenationinduced cardiomyocyte pyroptosis by regulating HMGB1 and NLRP3 inflammasome: L. Fei, et al.; Immunopharmacol. Immunotoxicol. 44, 500 (2022) [PMID: 35297734]
CARD-only proteins regulate in vivo inflammasome responses and ameliorate gout: S. Devi, et al.; Cell Rep. 42, 112265 (2023) [PMC10151391]
Exploring Candesartan, an angiotensin II receptor antagonist, as a novel inhibitor of NLRP3 inflammasome: alleviating inflammation in Neisseria gonorrhoeae infection: W.-Y. Lin, et al.; BMC Infect. Dis. 24, 1338 (2024) [PMC11585111]
Salmonella Enteritidis antitoxin DinJ inhibits NLRP3-dependent canonical inflammasome activation in macrophages: D. Gu, et al.; Infect. Immun. 92, e0050523 (2024) [PMC11003228]
In vivo imaging approach to detect and quantify early fibrogenic remodeling following low-dose bleomycin exposure: E. Mantovani, et al.; Front. Pharmacol. 17, 1792856 (2026) [PMC13102655]

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