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AdipoGen Life Sciences
anti-Caspase-1 (p20) (human), mAb (Bally-1) (Biotin)
As low as
500
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CHF 500.00
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AG-20B-0048B-C100100 µgCHF 500.00
Specifications / Handling
| Product Details | |
|---|---|
| Synonyms | Interleukin-1 β Convertase; IL-1BC; Interleukin-1 β-converting Enzyme; ICE |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | Bally-1 |
| Isotype | Mouse IgG1 |
| Source/Host | Purified from concentrated hybridoma tissue culture supernatant. |
| Immunogen/Antigen | Recombinant human caspase-1. |
| Label/Conjugates | Biotin |
| Application |
Western Blot (see online protocol): (1μg/ml) Note: Depending on the used cell line we recommend to optimize the dilution. |
| Crossreactivity | Human |
| Specificity |
Recognizes endogenous full-length and activated (p20 fragment) human caspase-1. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified. |
| Concentration | 0.5mg/ml |
| Formulation | Liquid. In PBS containing 0.02% sodium azide. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| Protocols |
 Download PDF |
| MSDS |
Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Scientific Background Information
Product Description
Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1β (IL-1β) and IL-18 and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called Inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3 responds to a variety of agonists including crystals.
Product-specific References
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- Caspase-11 cleaves gasdermin D for non-canonical inflammasome signaling: N. Kayagaki, et al.; Nature 526, 666 (2015) [PMID: 26375259]
- Human monocytes engage an alternative inflammasome pathway: M.M. Gaidt, et al.; Immunity 44, 833 (2016) [PMID: 27037191]
- Cell-Free Assay for Inflammasome Activation: Y. Jamilloux & F. Martinon; Methods Mol. Biol. 1417, 207 (2016) [PMID: 27221492]
- Modulation of Pathways Underlying Distinct Cell Death Mechanisms in Two Human Lung Cancer Cell Lines in Response to SN1 Methylating Agents Treatment: O. Papadodima, et al.; PLoS ONE 11, e0160248 (2016) [PMC4965087]
- The TAT-RasGAP317-326 anti-cancer peptide can kill in a caspase-, apoptosis-, and necroptosis-independent manner: M. Heulot, et al.; Oncotarget 7, 64342 (2016) [PMC5325447]
- Impact of human monocyte and macrophage polarization on NLR expression and NLRP3 inflammasome activation: F. Awad, et al.; PLoS ONE 12, e0175336 (2017) [PMC5389804]
- The DNA inflammasome in human myeloid cells is initiated by a STING-cell death program upstream of NLRP3: M.M. Gaidt, et al.; Cell 171, 1110 (2017) [PMC5901709]
- Calcium phosphate particles stimulate interleukin-1β release from human vascular smooth muscle cells: A role for spleen tyrosine kinase and exosome release: Y. Dautova, et al.; J. Mol. Cell Cardiol. 115, 82 (2018) [PMC5823844]
- POH1 deubiquitinates pro-interleukin-1β and restricts inflammasome activity: L. Zhang, et al.; Nat. Commun. 9, 4225 (2018) [PMC6185913]
- SUMO-mediated regulation of NLRP3 modulates inflammasome activity: R. Barry, et al.; Nat. Commun. 9, 3001 (2018) [PMC6070540]
- Hydrogen sulfide attenuates oxidative stress-induced NLRP3 inflammasome activation via S-sulfhydrating c-Jun at Cys269 in macrophages: Z. Lin, et al.; Biochim. Biophys. Acta 1864, 2890 (2018) [PMID: 29859240]
- The Crohn’s disease risk factor IRGM limits NLRP3 inflammasome activation by impeding its assembly and by mediating its selective autophagy: S. Mehto, et al.; Mol. Cell 73, 429 (2019) [PMC6372082]
- Human GBP1 is a microbe-specific gatekeeper of macrophage apoptosis and pyroptosis: D. Fisch, et al.; EMBO J. 38, e100926 (2019) [PMC6600649]
- HSP70 is a negative regulator of NLRP3 inflammasome activation: P. Martine, et al.; Cell Death Dis. 10, 256 (2019) [PMC6420651]
- Inflammasome Activation Triggers Blood Clotting and Host Death through Pyroptosis: C. Wu, et al.; Immunity 50, 1401 (2019) [PMC6791531]
- SERPINB1-mediated checkpoint of inflammatory caspase activation: Y.J. Choi, et al.; Nat. Immunol. 20, 276 (2019) [PMC6450391; Knock Down Validation]
- NLRP3 inflammasome activation drives tau pathology: C. Ising, et al.; Nature 575, 669 (2019) [PMC7324015]
- Human GBP1 Differentially Targets Salmonella and Toxoplasma to License Recognition of Microbial Ligands and Caspase-Mediated Death: D. Fisch, et al.; Cell Rep. 32, 108008 (2020) [PMC7435695]
- Molecularly Distinct NLRP3 Inducers Mediate Diverse Ratios of Interleukin-1β and Interleukin-18 from Human Monocytes: K. Midtbo, et al.; Mediators Inflamm. 2020, 4651090 (2020) [PMC7599400]
- Cross-regulation between LUBAC and caspase-1 modulates cell death and inflammation: T. Douglas & M. Saleh; J. Biol. Chem. 295, 5216 (2020) [PMC7170516]
- EphA2 phosphorylates NLRP3 and inhibits inflammasomes in airway epithelial cells: A. Zhang, et al.; EMBO Rep. 21, e49666 (2020) [PMC7332978]
- AIM2 Inhibits BRAF-Mutant Colorectal Cancer Growth in a Caspase-1-Dependent Manner: S. Shah, et al.; Front. Cell Dev. Biol. 9, 588278 (2021) [PMC8027362]
- Human NLRP1 is a sensor for double-stranded RNA: S. Bauernfried, et al.; Science 371, eabd0811 (2021) [PMID: 33243852]
- Post-injury immunosuppression and secondary infections are caused by an AIM2 inflammasome-driven signaling cascade: S. Roth, et al.; Immunity 54, 648 (2021) [PMID: 33667383]
- NLRP3 inflammasome activation in human peripheral blood mononuclear cells induced by venoms secreted PLA2s: M.D. Souza Silva, et al.; Int. J. Biol. Macromol. 202, 597 (2022) [PMID: 35074331]
- Crosstalk Between Pyroptosis and Apoptosis in Hepatitis C Virus-induced Cell Death: H.L. Wallace, et al.; Front. Immunol. 13, 788138 (2022) [PMC8882739]
- Optogenetic Induction of Pyroptosis, Necroptosis, and Apoptosis in Mammalian Cell Lines: K. Shkarina & P. Broz; Bio-protocol 13, e4762 (2023) [PMC10366993]
- HSP90β controls NLRP3 autoactivation: L. Spel, et al.; Sci. Adv. 10, eadj6289 (2024) [PMC10901362]
- Saikosaponin A targets HDAC6 to inhibit Mycobacterium tuberculosis-induced macrophage Pyroptosis via autophagy-mediated NLRP3 inflammasome inactivation: F. Liu, et al.; Phytomedicine 142, 156693 (2025) [PMID: 40393213]
- Lysosomal Cathepsin S Escape Facilitates Near Infrared Light-Triggered Pyroptosis Via an Antibody-Indocyanine Green Conjugate: F. Chen, et al.; Adv. Sci. 12, e04851 (2025) [PMC12442674]
- Vitamin B6 allosterically activates AMPK to promote postischemic angiogenesis in mice: X.Q. Wang, et al.; Eur. J. Pharmacol. 993, 177413 (2025) [PMID: 39986591]
- Evaluation of PANoptosis activation in irreversible pulpitis: An in vitro study based on human tissue samples: B. Yang, et al.; Arch. Oral Biol. 177, 106305 (2025) [PMID: 40451096]
- NLRP3-mediated glutaminolysis controls microglial phagocytosis to promote Alzheimer's disease progression: R.M. McManus, et al.; Immunity 58, 326 (2025) [PMID: 39904338]
- A cis-regulatory element controls expression of histone deacetylase 9 to fine-tune inflammasome-dependent chronic inflammation in atherosclerosis: Y. Asare, et al.; Immunity 58, 555 (2025) [PMID: 39879983]
- Inflammasome-independent IL-1β activation via staphopain A protease of Staphylococcus aureus: S. Bauernfried, et al.; J. Biol. Chem. 301, 110574 (2025) [PMC12528902]
- The NLRP6 inflammasome is activated by sterile or pathogen-induced endolysosomal damage: A. Boegli, et al.; EMBO J. 45, 30 (2026) [PMC12759077]
- Cofilin-1 is a Redox-Sensitive Guard of the NLRP3 Inflammasome: Y.H. Park, et al.; Nat. Immunol. 27, 937 (2026) [PMC13051731]
- Non-covalent mTOR inhibition suppresses NLRP3-GSDMD inflammasome-mediated pyroptosis: X. Jiang, et al.; Biochem. Pharmacol. 243, 117459 (2026) [PMID: 42619004]
- Neutrophil Inflammasome Activation and Pyroptosis Induced by a Metalloproteinase via HIF-1α and Gasdermin D Pathways: M.D.S. Silva, et al.; J. Innate Immun. 18, 169 (2026) [PMC13148814]
- GTPase Rab11b and effector Rab11-FIP2 promote NLRP3 stability during inflammasome priming: C.S. Gravastrand, et al.; EMBO J. 45, 2991 (2026) [PMC13144346]
- TRIM21 facilitates inflammasome assembly and contributes to autoinflammatory disease: J. Carriere, et al.; Nat. Commun. 17, 6726 (2026) [PMC13385917]
- The Neuroprotective Role of a Caspase-1 Inhibitor Against Apoptosis via Inhibition of Glial Hyperactivation in a Mouse Model of Epilepsy: H. Cai, et al.; J. Integr. Neurosci. 25, 49777 (2026) [PMID: 42216646]
- NLRP3 Inflammasome and Inflammation-Related Proteins Expression Characterized in Non-Cultivated Versus Cultivated Peripheral Blood Cells of Patients with Amyotrophic Lateral Sclerosis: D. Shevchuk, et al.; NeuroSci 7, 84 (2026) [PMC13516774]
- Nucleotide metabolic rewiring enables NLRP3 inflammasome hyperactivation in obesity: D. Liu, et al.; Science 391, eadq9006 (2026) [PMC13034487]
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