anti-GBP1 (human), pAb (IN111)

CHF 180.00
In stock
AG-25B-6001-C05050 µgCHF 180.00
More Information
Product Details
Synonyms Guanylate-binding Protein 1; GTP-binding Protein 1; Guanine Nucleotide-binding Protein 1; Interferon-induced Guanylate-binding Protein 1
Product Type Polyclonal Antibody
Properties
Source/Host Rabbit
Immunogen/Antigen Recombinant human GBP1 protein (aa 200-592).
Application

Western Blot: (1:500)*

*Note: We recommend starting a dilution of 1:500 and to optimize the dilution depending on your cells/tissues.

Crossreactivity Human
Specificity

Recognizes endogenous human GBP1. 

Purity ≥95% (SDS-PAGE)
Purity Detail Protein A affinity purified.
Concentration 1mg/ml
Formulation Liquid. In PBS containing 0.05% sodium azide.
Other Product Data

UniProt link P32455: GBP1 (human)

Accession Number P32455
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Guanylate-binding proteins (GBPs) are a family of antimicrobial proteins that are expressed by host cells in response to pathogens. Expression of GBPs is induced by both type I IFNs and IFN-γ. During infection with pathogen (S. Typhimurium or Salmonella), GBP1 binds first to surface of the bacteria, leading to the recruitment of GBP2, GBP3 and GBP4.  GBP1 binds to the component LPS (Lipopolysaccharide) of the bacteria as a first step bringing caspase-4 to the surface of bacteria, leading to caspase-1 activation, Gasdermin D cleavage and finally pyroptotic cell death. GBP1 is cleaved and inactivated by Caspase-1. GBP1 also promotes Toxoplasma DNA detection by AIM2-ASC-Caspase-8 leading to apoptosis. GBP1 acts as a gatekeeper of cell death pathways, which respond specifically to infecting microbes. 

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