AdipoGen Life Sciences

Z-Phe-Arg-AFC

CHF 70.00

In stock

AG-CP3-0028-M0055 mgCHF 70.00

AG-CP3-0028-M02525 mgCHF 280.00

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Product Details
Synonyms	Z-FR-AFC . TFA
Product Type	Chemical
Properties
Formula	C₃₃H₃₃F₃N₆O₆ . C₂HF₃O₂
MW	666.7 . 114.0
Sequence	Z-Phe-Arg-7-amino-4-trifluoromethylcoumarin
CAS	93753-73-2 (free base)
Purity Chemicals	≥95% (HPLC)
Appearance	White to off-white solid.
Solubility	Soluble in DMSO. Moderately soluble and highly stable in water-based solvents.
InChi Key	FLPBOIDNDJASFK-CCQIZPNASA-N
Smiles	[NH3+]C(NCCC[C@@H](C(NC1=CC=C(C(C(F)(F)F)=CC(O2)=O)C2=C1)=O)NC([C@H](CC3=CC=CC=C3)NC(OCC4=CC=CC=C4)=O)=O)=N.FC(F)(C([O-])=O)F
Shipping and Handling
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Keep cool and dry.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Fluorogenic substrate, specifically used to determine the enzyme activity of Cathepsin L.
Useful for the study of cathepsin activity and for screening small molecule inhibitors for drug discovery and HTS applications. Likely to be cleaved by cathepsins B, F, K, S and L, cathepsins L-like proteases and proteases such as papain, cruzipain, plasma kallikrein, soybean trypsin-like enzyme and the falcipains-1 and -2 (malaria hemoglobinases).
Hydrolysis of this substrate is monitored by observing fluorescence at an Excitation of 400nm and Emission at 505nm.

Product References

New fluorogenic substrates for alpha-thrombin, factor Xa, kallikreins, and urokinase: T. Morita, et al.; J. Biochem. 82, 1495 (1977)
Separation and identification of cathepsins in newborn rat epidermis: R.J. Harvima, et al.; J. Invest. Dermatol. 88, 393 (1987)
Preliminary studies on cysteine and serine proteinase activities in inflamed human gingiva using different 7-amino-4-trifluoromethyl coumarin substrates and protease inhibitors: S.W. Cox & B.M. Eley; Arch. Oral Biol. 32, 599 (1987)
Photometric or fluorometric assay of cathepsin B, L and H and papain using substrates with an aminotrifluoromethylcoumarin leaving group: J.R. Tchoupe, et al.; Biochim. Biophys. Acta 1076, 149 (1991)
Developmentally regulated secretion of cathepsin L-like cysteine proteases by Haemonchus contortus : M.L. Rhoads & R.H. Fetterer; J. Parasitol. 81, 505 (1995)
Taenia saginata Oncosphere Excretory/Secretory Peptidases: A. Clinton White, Jr. ; J. Parasitol. 82, 7 (1996)
Human cathepsin F: expression in baculovirus system, characterization and inhibition by protein inhibitors: M. Fonovic, et al.; Biol. Chem. 385, 505 (2004)
Identification and biochemical characterization of vivapains, cysteine proteases of the malaria parasite Plasmodium vivax : B.-K. NA, et al.; Biochem. J. 378, 529 (2004)
Crosstalk between the ubiquitin-proteasome system and autophagy in a human cellular model of Alzheimer's disease: V. Cecarini, et al.; Biochim. Biophys. Acta 1822, 1741 (2012)
Cysteine Protease Inhibitor (AcStefin) Is Required for Complete Cyst Formation of Acanthamoeba: J-Y. Lee, et al.; Eukaryot. Cell 12, 567 (2013)
Phospholamban overexpression in mice causes a centronuclear myopathy-like phenotype: V.A. Fajardo, et al.; Dis. Model Mech. 8, 999 (2015)
Data on skeletal muscle apoptosis, autophagy, and morphology in mice treated with doxorubicin: T.L. Campbell, et al.; Data Brief 7, 786 (2016)
Sarcolipin deletion exacerbates soleus muscle atrophy and weakness in phospholamban overexpressing mice: V.A. Fajardo, et al.; PLoS One 12, e0173708 (2017)

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