Cookie Policy: This site uses cookies to improve your experience. You can find out more about our use of cookies in our Privacy Policy. By continuing to browse this site you agree to our use of cookies.
AdipoGen Life Sciences
Boc-Val-Pro-Arg-AMC . HCl
90
CHF
CHF 90.00
In stock
AG-CP3-0038-M0055 mgCHF 90.00
AG-CP3-0038-M02525 mgCHF 360.00
Product Details | |
---|---|
Synonyms | Boc-VPR-AMC . HCl |
Product Type | Chemical |
Properties | |
Formula |
C31H45N7O7 . HCl |
MW | 627.7 . 36.5 |
Sequence |
Boc-Val-Pro-Arg-7-amido-4-methylcoumarin |
CAS | 70375-24-5 | 65147-04-8 (free base) |
Purity Chemicals | ≥98% |
Appearance | Solid lyophilized powder. |
Solubility | Soluble in water (10mg/ml). Soluble in 5% acetic acid. |
InChi Key | ROQGJTZGLVLVIN-UHFFFAOYSA-N |
Smiles | O=C1C=C(C)C2=CC=C(NC(C(CCCNC(N)=N)NC(C3N(C(C(NC(OC(C)(C)C)=O)C(C)C)=O)CCC3)=O)=O)C=C2O1.Cl |
Shipping and Handling | |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice |
Avoid freeze/thaw cycles. Protect from light. |
Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
Documents | |
MSDS | Download PDF |
Product Specification Sheet | |
Datasheet | Download PDF |
Description
Boc-Val-Pro-Arg-AMC is a sensitive fluorogenic peptide substrate for α-thrombin (Km = 21 μM, kcat = 109 s-1) and the α-thrombin-staphylocoagulase complex (Km = 25 μM, kcat = 89 s-1). It also serves as a substrate vor Kallikrein 5 and 8. Activity is quantified by the release of free fluorescent 7-amino-4-methylcoumarin (AMC) which excites at 360-380nm and emits at 440-460nm when interacting with a target enzyme. This substrate has also been used for the rapid detection of MRSA (methicillin-resistant Staphylococcus aureus).
Product References
- New fluorogenic substrates for alpha-thrombin, factor Xa, kallikreins, and urokinase: T. Morita, et al.; J. Biochem. 82, 1495 (1977)
- Difference in enzymatic properties between alpha-thrombin-staphylocoagulase complex and free alpha-thrombin: S. Kawabata, et al.; J. Biochem. 97, 1073 (1985)
- Evaluation of tube coagulase and a fluorogenic substrate for rapid detection of methicillin-resistant Staphylococcus aureus from selective enrichment broth in an outbreak of EMRSA 15: M. Ford, et al.; J. Hosp. Infect. 41, 133 (1999)
- Biochemical and Enzymatic Characterization of Human Kallikrein 5 (hK5), a Novel Serine Protease Potentially Involved in Cancer Progression: I.P. Michael, et al.; J. Biol. Chem. 280, 14628 (2005)
- Activation and enzymatic characterization of recombinant human kallikrein 8: T. Kishi, et al.; Biol. Chem. 387, 723 (2006)