Chemodex

2',7'-Dichlorofluorescein 3',6'-diacetate

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CDX-D0063-G0011 gCHF 75.00

CDX-D0063-G0055 gCHF 299.00

Specifications / Handling

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Product Details
Synonyms	2',7'-Dichlorofluorescein diacetate; DCFDA; Diacetyldichlorofluorescein; DCF-DA
Product Type	Chemical
Properties
Formula	C₂₄H₁₄Cl₂O₇
MW	485.27
CAS	2044-85-1
Source/Host Chemicals	Synthetic.
Purity Chemicals	≥97% (HPLC)
Appearance	White to off-white powder.
Solubility	Soluble in DMSO or acetone.
Identity	Determined by NMR.
Declaration	Manufactured by Chemodex.
Other Product Data	Click here for Original Manufacturer Product Datasheet Our product description may differ slightly from the original manufacturers product datasheet.
InChi Key	VQVUBYASAICPFU-UHFFFAOYSA-N
Smiles	CC(=O)OC1=C(Cl)C=C2C(OC3=CC(OC(C)=O)=C(Cl)C=C3C22OC(=O)C3=C2C=CC=C3)=C1
Shipping and Handling
Shipping	AMBIENT
Short Term Storage	+20°C
Long Term Storage	+4°C
Handling Advice	Keep cool and dry. Protect from light and moisture.
Use/Stability	Stable for at least 2 years after receipt when stored at +4°C.
Documents
Safety Data Sheet	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Scientific Background Information

Product Description

2',7'-Dichlorofluorescein diacetate (DCF-DA) is a diacetate-protected derivative of the fluorescent dye 2',7'-dichlorofluorescein (DCF). The acetate groups reduce fluorescence and increase membrane permeability. Following hydrolysis by intracellular or purified esterases, the acetate groups are removed and fluorescent 2',7'-dichlorofluorescein is generated. DCF-DA can therefore be used as a fluorogenic substrate for studying esterase activity and for applications in which enzymatic deacetylation to fluorescent DCF is monitored. Spectral data: λex=504nm, λem=524nm in 0.1 M Tris pH 8.0 (esterase).

Product-specific References

(1) A.R. Rosenkranz, et al.; J. Immunol. Meth. 156, 39 (1992) | (2) T. Takanashi, et al.; Invest. Ophthalmol. Vis. Sci. 38, 2721 (1997) | (3) E. Eruslanov & S. Kusmartsev; Methods Mol. Biol. 594, 57 (2010)

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