SBB-PS0006-M0022 mgCHF 131.00
20S Immunoproteasome vs. 20S Constitutive Proteasome Activity: Z-LLE-AMC exhibits a high specific activity and preference for 20S constitutive proteasome compared to 20S immunoproteasome.
|Synonyms||Proteasome Substrate II|
|Purity Chemicals||≥99% (HPLC)|
|Solubility||Soluble in DMSO.|
|Declaration||Manufactured by South Bay Bio.|
|Other Product Data||
Use: After preparing a stock solution in DMSO (≥10mM) store product at -20°C to -80°C. It is recommended to make multiple aliquots after the first thaw to ensure best performance. Caspase-like activity can be measured using a typical working concentration range from 10-50µM.
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Our product description may differ slightly from the original manufacturers product datasheet.
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
Avoid freeze/thaw cycles.
Protect from light.
|Use/Stability||Stable for at least 1 year after receipt when stored at -20°C.|
|Product Specification Sheet|
Z-LLE-AMC is a fluorogenic peptide substrate for measuring caspase-like activity of the proteasome. Hydrolysis of this substrate by the β1-subunit of the 20S proteasome is monitored by observing fluorescence at an Excitation wavelength of 345nm and Emission at 445nm. This substrate is specific to the constitutive proteasome and is not hydrolyzed efficiently by the immunoproteasome. 20S proteasome enzyme requires activation with 0.035% SDS in the assay buffer.
- 5,6,3',4'-Tetrahydroxy-7-methoxyflavone as a novel potential proteasome inhibitor: T.L. Chang, et al.; Planta Med. 76, 987 (2010)
- Characterization of a new series of non-covalent proteasome inhibitors with exquisite potency and selectivity for the 20S beta5-subunit: J. Blackburn, et al.; Biochem. J. 430, 461 (2010)