|Synonyms||Proteasome Substrate II|
|Purity Chemicals||≥95% (HPLC)|
|Solubility||Soluble in DMSO.|
|Declaration||Manufactured by South Bay Bio.|
|Other Product Data||
Use: After preparing a stock solution in DMSO (≥10mM) store product at -20°C to -80°C. It is recommended to make multiple aliquots after the first thaw to ensure best performance. Caspase-like activity can be measured using a typical working concentration range from 10-50µM. Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
Avoid freeze/thaw cycles.
Protect from light.
|Use/Stability||Stable for at least 1 year after receipt when stored at -20°C.|
|Product Specification Sheet|
Z-LLE-AMC is a fluorogenic peptide substrate for measuring caspase-like activity of the proteasome. Hydrolysis of this substrate by the β1-subunit of the 20S proteasome is monitored by observing fluorescence at an Excitation wavelength of 345nm and Emission at 445nm. This substrate is specific to the constitutive proteasome and is not hydrolyzed efficiently by the immunoproteasome. 20S proteasome enzyme requires activation with 0.035% SDS in the assay buffer.
- 5,6,3',4'-Tetrahydroxy-7-methoxyflavone as a novel potential proteasome inhibitor: T.L. Chang, et al.; Planta Med. 76, 987 (2010)
- Characterization of a new series of non-covalent proteasome inhibitors with exquisite potency and selectivity for the 20S beta5-subunit: J. Blackburn, et al.; Biochem. J. 430, 461 (2010)