Z-Leu-Leu-Glu-AMC [Z-LLE-AMC]

CHF 131.00
In stock
SBB-PS0006-M0022 mgCHF 131.00
 
More Information
Product Details
Synonyms Proteasome Substrate II
Product Type Chemical
Properties
Formula C35H44N4O9
MW 664.8
Sequence Z-Leu-Leu-Glu-7-amido-4-methylcoumarin
CAS 348086-66-8
Purity Chemicals ≥99% (HPLC)
Appearance Lyophilized powder.
Solubility Soluble in DMSO.
Declaration Manufactured by South Bay Bio.
Other Product Data Use: After preparing a stock solution in DMSO (≥10mM) store product at -20°C to -80°C. It is recommended to make multiple aliquots after the first thaw to ensure best performance. Caspase-like activity can be measured using a typical working concentration range from 10-50µM. Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer
Our product description may differ slightly from the original manufacturers product datasheet.
InChi Key FOYHOBVZPWIGJM-KCHLEUMXSA-N
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Protect from light.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF
Z-LLE-AMC is a fluorogenic peptide substrate for measuring caspase-like activity of the proteasome. Hydrolysis of this substrate by the β1-subunit of the 20S proteasome is monitored by observing fluorescence at an Excitation wavelength of 345nm and Emission at 445nm. This substrate is specific to the constitutive proteasome and is not hydrolyzed efficiently by the immunoproteasome. 20S proteasome enzyme requires activation with 0.035% SDS in the assay buffer.
Product References
  1. 5,6,3',4'-Tetrahydroxy-7-methoxyflavone as a novel potential proteasome inhibitor: T.L. Chang, et al.; Planta Med. 76, 987 (2010)
  2. Characterization of a new series of non-covalent proteasome inhibitors with exquisite potency and selectivity for the 20S beta5-subunit: J. Blackburn, et al.; Biochem. J. 430, 461 (2010)
© 2017 Adipogen Life Sciences. Pictures: © 2012 Martin Oeggerli. All Rights Reserved.