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SouthBayBio
Ac-Pro-Ala-Leu-AMC [Ac-PAL-AMC]
204
CHF
CHF 204.00
In stock
SBB-PS0007-M0022 mgCHF 204.00
20S Immunoproteasome vs. 20S Constitutive Proteasome Activity: Ac-PAL-AMC exhibits a high specific activity and preference for 20S immunoproteasome compared to constitutive 20S proteasome.
| Product Details | |
|---|---|
| Synonyms | Proteasome Substrate |
| Product Type | Chemical |
| Properties | |
| Formula |
C26H34N4O6 |
| MW | 498.6 |
| Sequence |
Acetyl-Pro-Ala-Leu-7-amido-4-methylcoumarin |
| CAS | 1431362-79-6 |
| Purity Chemicals | ≥95% (HPLC) |
| Appearance | Lyophilized powder. |
| Solubility | Soluble in DMSO. |
| Declaration | Manufactured by South Bay Bio. |
| Other Product Data |
Use: After preparing a stock solution in DMSO (≥10mM) store product at -20°C to -80°C. It is recommended to make multiple aliquots after the first thaw to ensure best performance. Caspase-like activity can be measured using a typical working concentration range from 10-50µM. Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer |
| InChi Key | BWJPVHDZSJFFDM-NDXORKPFSA-N |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
Avoid freeze/thaw cycles. Protect from light. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Ac-PAL-AMC is a fluorogenic peptide substrate for measuring caspase-like activity of the immunoproteasome. Hydrolysis of this substrate by the β1i-subunit of the immunoproteasome is monitored by observing fluorescence at an Excitation wavelength of 345nm and Emission at 445nm. This substrate is specific to the immunoproteasome and is not hydrolyzed efficiently by the constitutive proteasome.
Product References
- PSMB9 codon 60 polymorphisms have no impact on the activity of the immunoproteasome catalytic subunit B1i expressed in multiple types of solid cancer: J.E. Park, et al.; PloS one 8, e73732 (2013)
- Inhibitors of the immunoproteasome: current status and future directions: Z. Miller, et al.; Curr. Pharm. Des. 19, 4140 (2013)
- Development and characterization of selective immunoproteasome inhibitors: C. Dubiella; Diss. Universitaet Muenchen (2015)
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