SouthBayBio

Ac-Ala-Asn-Trp-AMC [Ac-ANW-AMC]

CHF 204.00

In stock

SBB-PS0009-M0022 mgCHF 204.00

20S Immunoproteasome vs. 20S Constitutive Proteasome Activity: Ac-ANW-ANW-AMC exhibits a specificity for 20S Immunoproteasome compared to 20S constitutive proteasome.

Specifications / Handling

More Information
Product Details
Synonyms	Proteasome Substrate
Product Type	Chemical
Properties
Formula	C₃₀H₃₂N₆O₇
MW	588.6
Sequence	Acetyl-Ala-Asn-Trp-7-amido-4-methylcoumarin
Purity Chemicals	≥95% (HPLC)
Appearance	Lyophilized powder.
Solubility	Soluble in DMSO.
Declaration	Manufactured by South Bay Bio.
Other Product Data	Use: After preparing a stock solution in DMSO (≥10mM) store product at -20°C to -80°C. It is recommended to make multiple aliquots after the first thaw to ensure best performance. Chymotrypsin-like activity can be measured using a typical working concentration range from 10-50µM. Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer Our product description may differ slightly from the original manufacturers product datasheet.
InChi Key	ORTKHFLJSJQDKO-ZCWWJEROSA-N
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Avoid freeze/thaw cycles. Protect from light.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS	Inquire
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Ac-ANW-AMC is a fluorogenic peptide substrate for measuring chymotrypsin-like activity of the immunoproteasome. Hydrolysis of this substrate by the β5i-subunit of the immunoproteasome is monitored by observing fluorescence at an Excitation wavelength of 345nm and Emission at 445nm. This substrate is specific to the immunoproteasome and is not hydrolyzed efficiently by the constitutive proteasome.

Product References

Elucidating the catalytic subunit composition of distinct proteasome subtypes: A crosslinking approach employing bifunctional activity-based probes: C.K. Cornish, et al.; ChemBioChem 16, 284 (2015)
Pharmacodynamic monitoring of (immuno) proteasome inhibition during bortezomib treatment of a critically ill patient with lupus nephritis and myocarditis: K.A. De Groot, et al.; Lupus Sci. Med. 2, e000121 (2015)
Immunoproteasome β5i-selective dipeptidomimetic inhibitors: P.K. Singh, et al.; ChemMedChem 11, 2127 (2016)
Acetaldehyde suppresses the display of HBV-MHC class I complexes on HBV-expressing hepatocytes: M. Ganesan, et al.; Am. J. Physiol. Gastrointest. Liver Physiol. 317, G127 (2019)
Monitoring the immunoproteasome in live cells using an activity-based peptide–peptoid hybrid probe: B.L. Zerfas & D.J. Trader; J. Am. Chem. Soc. 141, 5252 (2019)

Call	+41 61 926 6040
Fax	+41 61 926 6049