AdipoGen Life Sciences

anti-APRIL (mouse), mAb (blocking) (Centotto-1) (preservative free)

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AG-20B-0083PF-C100100 µgCHF 470.00
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Product Details
Synonyms A Proliferation Inducing Ligand; Tumor Necrosis Factor Ligand Superfamily Member 13; TNFSF13; TALL-2; CD256
Product Type Monoclonal Antibody
Properties
Clone Centotto-1
Isotype Mouse IgG1κ
Source/Host Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen Recombinant mouse APRIL.
Label/Conjugates Preservative Free
Application

Functional Application: Depletion of mouse APRIL. 
Immunoprecipitation: (1:200)

Crossreactivity Mouse
Specificity

Recognizes mouse recombinant and endogenous APRIL.

Purity ≥95% (SDS-PAGE)
Purity Detail Protein G-affinity purified.
Endotoxin Content <0.01EU/μg purified protein (LAL test).
Concentration 1mg/ml
Formulation Liquid. In PBS.
Isotype Negative Control

Mouse IgG1 Isotype Control (preservative free)

Other Product Data

Note: BAFF and APRIL contribute to maintaining IgG, IgA, and IgM plasma cells in the bone marrow (BM), spleen, and intestine and rely mainly on three ligand/receptor pairs: APRIL-BCMA, BAFF-TACI, and APRIL-TACI. Together, these actors account for 90% of circulating antibodies. To efficiently target plasma cells, dual inhibition of BAFF (using blocking anti-BAFF (mouse), mAb (Sandy-2) (Prod. No. AG-20B-0063)) and APRIL (using blocking anti-APRIL (mouse), mAb (blocking) (Centotto-1) (Prod. No. AG-20B-0083PF) both developed at AdipoGen Life Sciences are required in a recently published study (see also Figure).

Accession Number Q9D777
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

APRIL is a secreted cytokine, belonging to the TNF superfamily that binds to transmembrane activator and CAML interactor (TACI), B cell maturation antigen (BCMA) and can interact with carbohydrate side chains of heparan sulfate proteoglycans (HSPG) that may trigger cross-linking. APRIL maintains B cell homeostasis by acting at a later stage, modulating the function and survival of antigen-experienced B cells. APRIL (as well as BAFF) stimulates class-switch recombination (CSR), hence contributes to shaping humoral effector mechanisms. With regards to humoral memory, APRIL is involved in the establishment and survival of the long-lived plasma cell (LLPC) pool in the bone marrow (BM). Recently, APRIL has been shown to confer atheroprotection via binding to heparan sulfate (HS) chains of heparan-sulfate proteoglycan 2 (HSPG2), which limits LDL retention, macrophage accumulation and necrotic core formation. BAFF and APRIL are implicated in several human autoimmune diseases with autoreactive B cell involvement, including systemic lupus erythematosus (SLE), Sjögren’s syndrome (SS), IgA nephropathy (IgAN), and rheumatoid arthritis (RA). APRIL might also function in enhancing the proliferation of some tumor cells, especially B cell malignancies. BAFF and APRIL contribute to maintaining IgG, IgA, and IgM plasma cells in the bone marrow (BM), spleen and intestine and rely mainly on three ligand/receptor pairs: APRIL-BCMA, BAFF-TACI, and APRIL-TACI. APRIL plays an important role in B cell survival and proliferation, so blocking it can modulate immune responses. APRIL blocking antibodies can be useful for vaccine research, particularly to assess how APRIL inhibition affects normal immune responses to vaccination.

Product References
  1. APRIL limits atherosclerosis by binding to heparan sulfate proteoglycans: D. Tsiantoulas, et al.; Nature 597, 92 (2021)
  2. BAFF, but not APRIL, initiates Chronic Lymphocytic Leukemia by inducing tumor-promoting genes rather than cell survival: F. Mackay, et al.; ahead of print (2023)
  3. An unappreciated cell survival-independent role for BAFF initiating chronic lymphocytic leukemia: M.A. Ullah, et al.; Front. Immunol. 15, 1345515 (2024)
  4. Unique and redundant roles of mouse BCMA, TACI, BAFF, APRIL and IL-6 in supporting antibody-producing cells in different tissues: M. Eslami, et al.; PNAS 21, e2404309121 (2024)
© 2017 Adipogen Life Sciences. Pictures: © 2012 Martin Oeggerli. All Rights Reserved.