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AdipoGen Life Sciences
anti-APRIL (mouse), mAb (blocking) (Centotto-1) (preservative free)
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CHF 470.00
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AG-20B-0083PF-C100100 µgCHF 470.00
AG-20B-0083PF-C500500 µgCHF 1’100.00
Figure 1: In vivo depletion of gut IgA plasma cells by combined inhibition of BAFF and APRIL with the antibodies BAFF (mouse), mAb (blocking) (Sandy-2) (Prod. No. AG-20B-0063PF) and APRIL (mouse), mAb (blocking) (Centotto-1) (Prod. No. AG-20B-0083PF) in mice.
Method: A) Immunofluorescence of IgA-positive plasma cells (pink) and DAPI-positive nuclei (blue) in gut jejunum sections of 10-week-old wild type C57/Bl6 mice treated with 4 weekly i.p. injections of the indicated antibodies at 2 mg/kg. B) Quantification of IgA-positive plasma cells in the gut (average of 2 sections/mouse) of mice treated with Isotype control (n=5), or with anti-APRIL Centotto-1 (n=5), or with anti-BAFF Sandy-2 (n=4) or with both Centotto-1 and Sandy-2 (n=5). One way ANOVA with Dunnett’s multiple comparison test. ns: not significant. **** P<0.0001. Picture courtesy of Dr Mahya Eslami, Dr Pascal Schneider Lab, University of Lausanne, Switzerland
Method: A) Immunofluorescence of IgA-positive plasma cells (pink) and DAPI-positive nuclei (blue) in gut jejunum sections of 10-week-old wild type C57/Bl6 mice treated with 4 weekly i.p. injections of the indicated antibodies at 2 mg/kg. B) Quantification of IgA-positive plasma cells in the gut (average of 2 sections/mouse) of mice treated with Isotype control (n=5), or with anti-APRIL Centotto-1 (n=5), or with anti-BAFF Sandy-2 (n=4) or with both Centotto-1 and Sandy-2 (n=5). One way ANOVA with Dunnett’s multiple comparison test. ns: not significant. **** P<0.0001. Picture courtesy of Dr Mahya Eslami, Dr Pascal Schneider Lab, University of Lausanne, Switzerland
| Product Details | |
|---|---|
| Synonyms | A Proliferation Inducing Ligand; Tumor Necrosis Factor Ligand Superfamily Member 13; TNFSF13; TALL-2; CD256 |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | Centotto-1 |
| Isotype | Mouse IgG1κ |
| Source/Host | Purified from concentrated hybridoma tissue culture supernatant. |
| Immunogen/Antigen | Recombinant mouse APRIL. |
| Label/Conjugates | Preservative Free |
| Application |
Functional Application: Depletion of mouse APRIL. |
| Crossreactivity | Mouse |
| Specificity |
Recognizes mouse recombinant and endogenous APRIL. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified. |
| Endotoxin Content | <0.01EU/μg purified protein (LAL test). |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS. |
| Isotype Negative Control | |
| Other Product Data |
Note: BAFF and APRIL contribute to maintaining IgG, IgA, and IgM plasma cells in the bone marrow (BM), spleen, and intestine and rely mainly on three ligand/receptor pairs: APRIL-BCMA, BAFF-TACI, and APRIL-TACI. Together, these actors account for 90% of circulating antibodies. To efficiently target plasma cells, dual inhibition of BAFF (using blocking anti-BAFF (mouse), mAb (Sandy-2) (Prod. No. AG-20B-0063)) and APRIL (using blocking anti-APRIL (mouse), mAb (blocking) (Centotto-1) (Prod. No. AG-20B-0083PF) both developed at AdipoGen Life Sciences are required in a recently published study (see also Figure). |
| Accession Number | Q9D777 |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
APRIL is a secreted cytokine, belonging to the TNF superfamily that binds to transmembrane activator and CAML interactor (TACI), B cell maturation antigen (BCMA) and can interact with carbohydrate side chains of heparan sulfate proteoglycans (HSPG) that may trigger cross-linking. APRIL maintains B cell homeostasis by acting at a later stage, modulating the function and survival of antigen-experienced B cells. APRIL (as well as BAFF) stimulates class-switch recombination (CSR), hence contributes to shaping humoral effector mechanisms. With regards to humoral memory, APRIL is involved in the establishment and survival of the long-lived plasma cell (LLPC) pool in the bone marrow (BM). Recently, APRIL has been shown to confer atheroprotection via binding to heparan sulfate (HS) chains of heparan-sulfate proteoglycan 2 (HSPG2), which limits LDL retention,macrophage accumulation and necrotic core formation.
Product References
- APRIL limits atherosclerosis by binding to heparan sulfate proteoglycans: D. Tsiantoulas, et al.; Nature 597, 92 (2021)
- BAFF, but not APRIL, initiates Chronic Lymphocytic Leukemia by inducing tumor-promoting genes rather than cell survival: F. Mackay, et al.; ahead of print (2023)
- An unappreciated cell survival-independent role for BAFF initiating chronic lymphocytic leukemia: M.A. Ullah, et al.; Front. Immunol. 15, 1345515 (2024)
- Unique and redundant roles of mouse BCMA, TACI, BAFF, APRIL and IL-6 in supporting antibody-producing cells in different tissues: M. Eslami, et al.; PNAS 21, e2404309121 (2024)
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