AdipoGen Life Sciences

IL-36Ra (mouse) (monomer):Fc (silent) InVivoKine™

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AG-40B-0285-C05050 µgCHF 440.00

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Figure 1: Protein structure of IL-36Ra (mouse) (monomer):Fc (silent) InVivoKine™ (Prod. No. AG-40B-0285).

Specifications / Handling

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Product Details
Synonyms	Interleukin-36Ra (mouse):Fc (LALA-PG) KIH (human) (rec.); Interleukin-36 Receptor Antagonist Protein; IL-1F5; Interleukin-1 Family Member 5; IL-1δ; Interleukin-1δ; Interleukin-1 Homolog 3; Interleukin-1-like
Product Type	Protein
Properties
Source/Host	HEK 293 cells. Produced using animal component-free medium.
Sequence	Mouse IL-36Ra (aa 3-156) is fused at the C-terminus to the Fc portions of human IgG1 (LALA-PG) (Knobs-into-Holes technology) (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)).
Crossreactivity	Mouse
Specificity	The Fc contains the mutations LALA-PG that abolish the interaction between the Fc and FcγRs and therefore Fc undesirable effects. The Fc (LALA-PG) is named Fc (silent).
MW	~ 52 kDa and ~ 28kDa (SDS-PAGE)
Purity	≥95% (SDS-PAGE)
Endotoxin Content	<0.01 EU/μg protein (LAL test).
Concentration	After reconstitution: 1mg/ml
Reconstitution	Reconstitute with 50μl endotoxin-free water.
Accession Number	Q9QYY1
Formulation	Lyophilized. Contains PBS.
Protein Negative Control	Fc (silent) InVivoKine™ (human) IgG1 Control (Prod. No. AG-35B-0018)
Other Product Data	Uniprot Q9QYY1: Interleukin-36 receptor antagonist protein
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

IL-36Ra/IL-1F5 is a highly and a specific antagonist of the IL-1 receptor-related protein 2-mediated response to IL-36α (IL-1F6), IL-36β (IL-1F8) and IL-36γ (IL-1F9). These cytokines bind to IL-36R (IL-1Rrp2) and IL-1RAcP, activating similar intracellular signals as IL-1. IL-36Ra inhibits the production of proinflammatory cytokines, including IL-12, IL-1β, IL-6, TNF-α and IL-23 induced by IL-36 in BMDC and CD4 T cells. Skin and dendritic cells are target of the IL-36 interleukins leading to a Th1 response. Recently, mutations that affect the levels and the activity of IL-36Ra have been found in patients with pustular psoriasis, leading to enhanced production of inflammatory cytokines (IL-8 in particular) by keratinocytes.

The IL-36Ra (mouse):Fc (silent) InVivoKine™ is produced by using two different vectors, one encoding for the IL-36Ra (mouse):Fc (LALA-PG) Knobs sequence (synthesizing a protein of 52 kDa) and one encoding for the Fc (LALA-PG) Holes sequence (synthesizing a protein of 28kDa). Both vectors transfected into HEK293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization of the Fc moieties and for secretion of the final protein IL-36Ra (mouse) (monomeric):Fc (silent) InVivoKine™. The LALA-PG mutations inhibit binding to FcγRs and C1q while FcRn binding and Fc stability remain unaffected.

InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical and translational in vivo research, developed and manufactured by AdipoGen Life Sciences.

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