Erythroferrone (human) ELISA Kit

CHF 690.00
In stock
AG-45B-0014-KI0196 wellsCHF 690.00
More Information
Product Details
Synonyms CTRP15; Complement C1q Tumor Necrosis Factor-related Protein 15; Myonectin; FAM132B; ERFE
Product Type Kit
Properties
Application Set Quantitative ELISA
Specificity

Detects human Erythroferrone [Myonectin; CTRP15] in serum, plasma and cell culture supernatant.

Crossreactivity Human
Quantity

1 x 96 wells

Sensitivity 270pg/ml
Range 0.3125 to 20ng/ml
Sample Type Cell Culture Supernatant
Plasma
Serum
Assay Type Sandwich
Detection Type Colorimetric
Other Product Data

UniProt link Q4G0M1: Erythroferrone (human)

See our Erythroferrone Landing Page for important Information!

Accession Number Q4G0M1
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice After standard reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Plate and reagents should reach room temperature before use.
Use/Stability 12 months after the day of manufacturing. See expiry date on ELISA Kit box.
Documents
Manual Download PDF
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Erythroferrone (also called Myonectin, CTRP15 or Fam13B) is a member of CTRPs (C1q/TNF-related proteins), which are conserved paralogs of adiponectin containing collagen-like and globular C1q-like domains. Erythroferrone plays two different regulatory roles:

i) Erythroferrone (called Myonectin for this role) is a myokine abundantly expressed in skeletal muscle tissue, upregulated by voluntary exercise that is suppressed by fasting and induced by refeeding; it participates in the regulation of systemic lipid metabolism by promoting the clearance of nonesterified fatty acids (NEFA) from circulation. As a myokine, Erythroferrone/Myonectin acts as an endurance exercise-driven myokine which protects the heart from ischemic injury by reducing cardiomyocyte apoptosis and macrophage inflammatory response, and

ii) Erythroferrone is produced by erythroblasts after bleeding or EPO treatment and acts on hepatocytes to suppress hepcidin expression, resulting in increased release of iron from cellular iron stores. Erythroferrone acts on hepcidin by inhibiting members of the BMP-5, BMP-6 and BMP-7 subgroup of BMPs. Erythroferrone functions as erythroid modulator of iron metabolism and hemoglobin synthesis.

Levels of Erythroferrone measured by this ELISA Kit in serum and plasma correspond to mouse protein levels (~500ng/ml). Our Erythroferrone assay has been validated by the Swiss Laboratory for Doping Analyses in a recent publication, reporting that our assay can sensitively detect the stimulation of erythropoiesis, including erythropoiesis stimulating agents (ESA, such as Erythropoietin) abuse and blood withdrawal. Erythroferrone is a biomarker for different types of anemia, for cardiology and potentially also for metabolic diseases. 

Product References
  1. Erythroferrone as a sensitive biomarker to detect stimulation of erythropoiesis: K. Ramirez Cuevas, et al.; Drug Test. Anal. (Epub ahead of print) (2019) >>> The same Antibody Matched Pair is used in this publication as in our ELISA Kit!
© 2017 Adipogen Life Sciences. Pictures: © 2012 Martin Oeggerli. All Rights Reserved.