AdipoGen Life Sciences

H-D-Val-Leu-Lys-pNA . 2HCl

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Specifications / Handling

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Product Details
Synonyms	H-D-Valyl-L-leucyl-L-lysine-p-Nitroanilide . 2HCl; vLK-pNA
Product Type	Chemical
Properties
Formula	C₂₃H₃₈N₆O₅ . 2HCl
MW	478.6 . 72.9
Sequence	D-Val-Leu-Lys-p-Nitroanilide
CAS	62354-43-2
Purity Chemicals	≥98% (HPLC)
Appearance	White to off-white solid.
Solubility	Soluble in water (50mg/ml).
InChi Key	VESQMNNSPPEOSZ-ZLARAOTRSA-N
Smiles	[H]Cl.[H]Cl.O=C(N[C@@H](CCCCN)C(NC1=CC=C([N+]([O-])=O)C=C1)=O)[C@H](CC(C)C)NC([C@@H](C(C)C)N)=O
Shipping and Handling
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Keep cool and dry.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product Description

D-Val-Leu-Lys-p-nitroanilide is a selective colorimetric substrate for plasmin used to determine the formation of plasmin from plasminogen in amidolytic activity assays and plasminogen activating assays. Plasmin is a plasma serine protease whose main role is to dissolve fibrin blood clots.
After cleavage by plasmin the protease activity is quantified by the release of p-nitroaniline (pNA) from the substrate. The rate of pNA formation, e.g. the increase in absorbance per second at 405 nm, is proportional to the enzymatic activity.

Product-specific References

Chromogenic substrates: Scan. J. Clin. Lab. Invest. 42, 15 (1982)
Plasminogen Activation in Diabetes Mellitus: M. Geiger & B.R. Binder; J. Biol. Chem. 259, 2976 (1984)
Purification and characterization of a fibrinolytic enzyme of Bacillus subtilis DC33, isolated from Chinese traditional Douchi: C.T. Wang, et al.; J. Ind. Microbiol. Biotechnol. 33, 750 (2006)
Regulation of plasminogen activator activity and expression by cyclic mechanical stress in rat mandibular condylar chondrocytes: W. Chen, et al.; Mol. Med. Rep. 8, 1155 (2013)
Purifcation and characterization of a novel, highly potent fbrinolytic enzyme from Bacillus subtilis DC27 screened from Douchi, a traditional Chinese fermented soybean food: Y. Hu, et al.; Sci. Rep. 9, 9235 (2019)
Heat-Treatments Affect Protease Activities and Peptide Profiles of Ruminants' Milk: J.A.S. Leite, et al.; Front. Nutr. 8, 626475 (2021)

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