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AdipoGen Life Sciences
H-D-Val-Leu-Lys-pNA . 2HCl
70
CHF
CHF 70.00
In stock
AG-CP3-0042-M0055 mgCHF 70.00
AG-CP3-0042-M02525 mgCHF 280.00
| Product Details | |
|---|---|
| Synonyms | H-D-Valyl-L-leucyl-L-lysine-p-Nitroanilide . 2HCl; vLK-pNA |
| Product Type | Chemical |
| Properties | |
| Formula |
C23H38N6O5 . 2HCl |
| MW | 478.6 . 72.9 |
| Sequence |
D-Val-Leu-Lys-p-Nitroanilide |
| CAS | 62354-43-2 |
| Purity Chemicals | ≥98% (HPLC) |
| Appearance | White to off-white solid. |
| Solubility | Soluble in water (50mg/ml). |
| InChi Key | VESQMNNSPPEOSZ-ZLARAOTRSA-N |
| Smiles | [H]Cl.[H]Cl.O=C(N[C@@H](CCCCN)C(NC1=CC=C([N+]([O-])=O)C=C1)=O)[C@H](CC(C)C)NC([C@@H](C(C)C)N)=O |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Keep cool and dry. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
- D-Val-Leu-Lys-p-nitroanilide is a selective colorimetric substrate for plasmin used to determine the formation of plasmin from plasminogen in amidolytic activity assays and plasminogen activating assays. Plasmin is a plasma serine protease whose main role is to dissolve fibrin blood clots.
- After cleavage by plasmin the protease activity is quantified by the release of p-nitroaniline (pNA) from the substrate. The rate of pNA formation, e.g. the increase in absorbance per second at 405 nm, is proportional to the enzymatic activity.
Product References
- Chromogenic substrates: Scan. J. Clin. Lab. Invest. 42, 15 (1982)
- Plasminogen Activation in Diabetes Mellitus: M. Geiger & B.R. Binder; J. Biol. Chem. 259, 2976 (1984)
- Purification and characterization of a fibrinolytic enzyme of Bacillus subtilis DC33, isolated from Chinese traditional Douchi: C.T. Wang, et al.; J. Ind. Microbiol. Biotechnol. 33, 750 (2006)
- Regulation of plasminogen activator activity and expression by cyclic mechanical stress in rat mandibular condylar chondrocytes: W. Chen, et al.; Mol. Med. Rep. 8, 1155 (2013)
- Purifcation and characterization of a novel, highly potent fbrinolytic enzyme from Bacillus subtilis DC27 screened from Douchi, a traditional Chinese fermented soybean food: Y. Hu, et al.; Sci. Rep. 9, 9235 (2019)
- Heat-Treatments Affect Protease Activities and Peptide Profiles of Ruminants' Milk: J.A.S. Leite, et al.; Front. Nutr. 8, 626475 (2021)
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