BellBrook

Enzolution™ DHX9 ATPase TR-FRET Assay System

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In stock
BBL-3042-TR-1K1000 assaysCHF 671.00
BBL-3042-TR-10K10000 assaysCHF 3'076.00
BBL-3042-TR-100K10 x 10000 assaysINQ
NEW
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Product Details
Synonyms ATP-dependent RNA Helicase A; DEAH Box Protein 9; Leukophysin
Product Type Kit
Properties
Application Set Compound Screening
Crossreactivity Human
Quantity

1000 assays/384wells (BBL-3042-TR-1K)

10000 assays/384wells (BBL-3042-TR-10K)

100000 assays/384wells (BBL-3042-TR-100K)

Note: The exact number of assays depends on enzyme reaction conditions.

Detection Type TR-FRET
Kit Contains

- DHX9 (human) (rec.) (His) Enzyme
- Yeast RNA (10mg/ml)
- Enzyme Assay Buffer E, 10X
- 384-Well Low Volume Assay Plates (Corning #4513; sold under #BBL-2231 or BBL-2232)

Other Product Data

Click here for Complete Information from the Original Manufacturer
Our product description may differ slightly from the original manufacturers product datasheet.

Declaration Manufactured by BellBrook Labs.
Accession Number Q08211
Shipping and Handling
Shipping DRY ICE
Short Term Storage -20°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Documents
Manual Download PDF
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF
Description

The Enzolution™ DHX9 ATPase Assay System is intended for use with the Transcreener® ADP2 Assay Kits to measure the ATP-dependent RNA helicase activity of DHX9, a member of the a DExH/D helicase family. Though it is classified as an RNA helicase, DHX9 also has been reported to unwind DNA and DNA:RNA hybrids. ADP formation by the RNA helicase activity of DHX9 can be easily measured by the Transcreener ADP2 assay, a far-red, competitive fluorescence assay that enables single addition, mix-and-read detection in a continuous or endpoint format. The assay has been optimized and extensively validated for high throughput screening (HTS) and inhibitor dose response measurements using most multimode plate readers. The Transcreener ADP TR-FRET Assay is not included in the Enzolution Assay System but is available for separate purchases. The Transcreener ADP assay is designed for HTS, Hit-to-Lead, SAR, and general discovery.

When purchasing the DHX9 ATPase Assay System, it is important to choose the assay system readout corresponding to the TR-FRET configuration of your Transcreener ADP2 Assay Kit!

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