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Items 31-40 of 64

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  1. M1-linked Tetra-Ubiqutin (human) (rec.) (untagged)
    SBB-UP0119
    SBB-UP0119-C02525 µg
    CHF 287.00
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  2. MDM2 E3 Ligase TR-FRET Kit
    SBB-KF0030
    SBB-KF0030-KI011 Kit
    CHF 1’286.00
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    <b>% Signal to Background of Continuous Real-Time TR-FRET MDM2 titration (autoubiquitination):</b> Serial dilutions of GST-MDM2 from 200nM to 12.5nM mixed with UBA1, UBE2D3 and trf-Ub mix. Reaction was initiated with addition of Mg-ATP.
  3. Nedd4 E3 Ligase TR-FRET Kit
    SBB-KF0056
    SBB-KF0056-KI011 Kit
    CHF 1’286.00
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    % Signal to Background of Continuous Real-Time TR-FRET NEDD4 titration (autoubiquitination): Serial dilutions of NEDD4 from 50nM to 3.125nM mixed with UBA1, UBE2L3, and, TRF-Ub mix. Reaction was initiated with addition of Mg-ATP.
  4. NEDD8 (human) (rec.) (Rhodamine 110)
    SBB-PS0003
    SBB-PS0003-C05050 µg
    CHF 447.00
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    <b>Signal to Background:</b> The signal to background ratio was determined by 100% hydrolysis of 800nM, 400nM, 200nM, 100nM, 50nM NEDD8-Rhodamine 110 to liberate the quenched conjugate. Assay Buffer: 50mM HEPES pH7.5, 1mM TCEP, 0.1mg/ml BSA.
  5. NEDD8-AMC (human) (rec.) (untagged)
    SBB-PS0044
    SBB-PS0044-C05050 µg
    CHF 370.00
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    <b>Robustness of NEDD8-AMC substrates in an HTS format:</b> Fluorescent substrate NEDD8-AMC was incubated with and without 30pM NEDP1 in a 384 well plate (n = 16), and progress curves were normalized to the maximum fluorescence signal to produce “% reacti
  6. NEDP1 [SENP8] (human) (rec.) (His)
    SBB-DE0025
    SBB-DE0025-C05050 µg
    CHF 248.00
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    <b>Michaelis–Menten Kinetics:</b> NEDD8 Rhodamine 110 serially diluted from 1.6 to 0.0125µM was digested with 30pM NEDP1 over time. The assay was carried out in a reaction buffer of 50mM HEPES pH 7.5, 1mM TCEP, 0.1 mg/ml BSA, at 25°C. Initial velocities a
  7. p53 (polyubiquitinated) (human) (rec.) (His)
    SBB-US0012
    SBB-US0012-C02020 µg
    CHF 660.00
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    <b>Ubiquitinated-p53 Western Blot:</b> From left to right: Control His6-p53 (50ng), ubiquitinated-p53 (50ng and 100ng) and ubiquitinated-p53 digested with USP7 (50ng).
  8. PA28alpha (human) (rec.) (untagged)
    SBB-PP0080
    SBB-PP0080-C100100 µg
    CHF 345.00
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    PA28alpha (human) (rec.) (untagged)
  9. Parkin (E3 Ligase) (human) (rec.) (His) (Biotin)
    SBB-CE0107
    SBB-CE0107-C02525 µg
    CHF 573.00
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    Biotin-Parkin SDS-PAGE. From left to right, increasing amounts of His6-Parkin were loaded onto a 4-20% SDS-PAGE gel and stained with coomassie brillant blue. Purity is >95%.
  10. Parkin (E3 Ligase) (human) (rec.) (His)
    SBB-CE0055
    SBB-CE0055-C05050 µg
    CHF 573.00
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    His<sub>6</sub>- Parkin SDS-PAGE. From left to right, increasing amounts of His<sub>6</sub>- Parkin (Prod. No. SBB-CE0055) were loaded onto a 4-20% SDS-PAGE gel and stained with coomassie brillant blue.

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