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Items 31-40 of 53

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  1. Nedd4 E3 Ligase TR-FRET Kit
    SBB-KF0056
    SBB-KF0056-KI011 Kit
    CHF 1'250.00
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    % Signal to Background of Continuous Real-Time TR-FRET Parkin titration (autoubiquitination): Serial dilutions of Parkin W403A from 50nM to 3.125nM and 300nM wt Parkin were mixed with UBA1, UBE2D3 and trf-Ub (pS65) mix. Reactions were initiated wit
  2. NEDD8 (human) (rec.) (Rhodamine 110)
    SBB-PS0003
    SBB-PS0003-C05050 µg
    CHF 434.00
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    Signal to Background: The signal to background ratio was determined by 100% hydrolysis of 800nM, 400nM, 200nM, 100nM, 50nM NEDD8-Rhodamine 110 to liberate the quenched conjugate. Assay Buffer: 50mM HEPES pH7.5, 1mM TCEP, 0.1mg/ml BSA.
  3. NEDD8-AMC (human) (rec.) (untagged)
    SBB-PS0044
    SBB-PS0044-C05050 µg
    CHF 360.00
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    NEDD8-AMC (human) (rec.) (untagged)
  4. NEDP1 [SENP8] (human) (rec.) (His)
    SBB-DE0025
    SBB-DE0025-C05050 µg
    CHF 243.00
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    Michaelis–Menten Kinetics: NEDD8 Rhodamine 110 serially diluted from 1.6 to 0.0125µM was digested with 30pM NEDP1 over time. The assay was carried out in a reaction buffer of 50mM HEPES pH 7.5, 1mM TCEP, 0.1 mg/ml BSA, at 25°C. Initial velocities a
  5. p53 (polyubiquitinated) (human) (rec.) (His)
    SBB-US0012
    SBB-US0012-C02020 µg
    CHF 639.00
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    Ubiquitinated-p53 Western Blot: From left to right: Control His6-p53 (50ng), ubiquitinated-p53 (50ng and 100ng) and ubiquitinated-p53 digested with USP7 (50ng).
  6. Parkin (E3 Ligase) (human) (rec.) (His)
    SBB-CE0055
    SBB-CE0055-C05050 µg
    CHF 557.00
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    Parkin (E3 Ligase) (human) (rec.) (His)
  7. Parkin E3 Ligase TR-FRET Kit
    SBB-KF0036
    SBB-KF0036-KI011 Kit
    CHF 1'250.00
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    Parkin E3 Ligase TR-FRET Kit
  8. Suc-Leu-Leu-Val-Tyr-AMC [Suc-LLVY-AMC]
    CAS  94367-21-2
    SBB-PS0010
    SBB-PS0010-M0022 mg
    CHF 101.00
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    Suc-Leu-Leu-Val-Tyr-AMC [Suc-LLVY-AMC]
  9. TRF-Ubiquitin Mix (100X) (2000 reactions)
    SBB-TR0051-2K
    SBB-TR0051-2K-11 Vial
    CHF 5'255.00
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    TRF-Ubiquitin Mix (100X) (2000 reactions)
  10. TRF-Ubiquitin Mix (100X) (400 reactions)
    SBB-TR0051-4H
    SBB-TR0051-4H-11 Vial
    CHF 1'233.00
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    TRF-Ubiquitin Mix (100X) (400 reactions)

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