SouthBayBio

Suc-Leu-Leu-Val-Tyr-AMC [Suc-LLVY-AMC]

CHF 101.00

In stock

SBB-PS0010-M0022 mgCHF 101.00

20S Immunoproteasome vs. 20S Constitutive Proteasome Activity: Suc-LLVY-AMC exhibits a high specific activity for both immuno and constitutive proteasome compared to other substrates. Specific activities are higher when using immunoproteasome.

Specifications / Handling

More Information
Product Details
Synonyms	Proteasome Substrate
Product Type	Chemical
Properties
Formula	C₄₀H₅₃N₅O₁₀
MW	763.9
Sequence	N-Succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin
CAS	94367-21-2
Purity Chemicals	≥95% (HPLC)
Appearance	Lyophilized powder.
Solubility	Soluble in DMSO.
Declaration	Manufactured by South Bay Bio.
Other Product Data	Use: After preparing a stock solution in DMSO (≥10mM) store product at -20°C to -80°C. It is recommended to make multiple aliquots after the first thaw to ensure best performance. Chymotrypsin-like activity can be measured using a typical working concentration range from 10-50µM. Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer Our product description may differ slightly from the original manufacturers product datasheet.
InChi Key	UVFAEQZFLBGVRM-FEIGSSFXSA-N
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Avoid freeze/thaw cycles. Protect from light.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS	Inquire
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Suc-LLVY-AMC is a fluorogenic substrate for measuring the chymotrypsin-like peptidase activity of the 20S and 26S proteasome, calpains and other chymotrypsin-like proteases. Cleavage of the peptide by the enzymes liberates the fluorophore AMC which generates strong increase in fluorescence fluorescent that can be monitored fluorimetrically.
Excitation=345nm
Emission=445nm.

Product References

Comparative specificity and kinetic studies on porcine calpain I and calpain II with naturally occurring peptides and synthetic fluorogenic substrates: T. Sasaki, et al.; J. Biol. Chem. 259, 12489 (1984)
Kinetic characterization of the chymotryptic activity of the 20S proteasome: R.L. Stein, et al.; Biochemistry 35, 3899 (1996)
26S proteasome activity is down-regulated in lung cancer stem-like cells propagated in vitro: J. Pan, et al.; PLoS One 5, e13298 (2010)
Low cell density induced epithelial-mesenchymal transition in MCF10A cell is regulated by proteasome activity: J. Fu, et al.; J. Oncobiomark. 1, 4 (2013)
Marchantin M: a novel inhibitor of proteasome induces autophagic cell death in prostate cancer cells: H. Jiang, et al.; Cell Death Dis. 4, e761 (2013)

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