Erythroferrone (human) Matched Pair Detection Set
|Synonyms||CTRP15; Complement C1q Tumor Necrosis Factor-related Protein 15; Myonectin; FAM132B; ERFE|
|Application Set||Quantitative ELISA|
Detects human Erythroferrone [Myonectin; CTRP15] in serum, plasma and cell culture supernatant.
Contains sufficient materials to run ELISAs on 5 x 96-well plates.
|Range||0.078 to 5ng/ml|
Cell Culture Supernatant
1 vial standard protein (100ng) (lyophilized) [STD]
1 vial coating antibody (120µl) [COAT]
1 vial detection antibody (60µl) [DET]
1 vial streptavidin-HRP (10µg) (lyophilized) [STREP]
|Other Product Data||
UniProt link Q4G0M1: Erythroferrone (human)
|Shipping and Handling|
|Short Term Storage||-20°C|
|Long Term Storage||-20°C|
After standard reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
|Use/Stability||Stable for at least 1 year after receipt when stored at -20°C.|
|Product Specification Sheet|
Erythroferrone (also called Myonectin, CTRP15 or Fam13B) is a member of CTRPs (C1q/TNF-related proteins), which are conserved paralogs of adiponectin containing collagen-like and globular C1q-like domains. Erythroferrone plays two different regulatory roles:
i) Erythroferrone (called Myonectin for this role) is a myokine abundantly expressed in skeletal muscle tissue, upregulated by voluntary exercise that is suppressed by fasting and induced by refeeding; it participates in the regulation of systemic lipid metabolism by promoting the clearance of nonesterified fatty acids (NEFA) from circulation. As a myokine, Erythroferrone/Myonectin acts as an endurance exercise-driven myokine which protects the heart from ischemic injury by reducing cardiomyocyte apoptosis and macrophage inflammatory response.
ii) Erythroferrone is produced by erythroblasts after bleeding or EPO treatment and acts on hepatocytes to suppress hepcidin expression, resulting in increased release of iron from cellular iron stores. Erythroferrone acts on hepcidin by inhibiting members of the BMP-5, BMP-6 and BMP-7 subgroup of BMPs. Erythroferrone functions as erythroid modulator of iron metabolism and hemoglobin synthesis.
Our Erythroferrone (human) Matched Pair Detection Set has been validated by the Swiss Laboratory for Doping Analyses in a recent publication reporting that our assay can sensitively detect the stimulation of erythropoiesis, including erythropoiesis stimulating agents (ESA, such as Erythropoietin) abuse and blood withdrawal. Erythroferrone is a biomarker for different types of anemia, for cardiology and potentially also for metabolic diseases.
- Erythroferrone as a sensitive biomarker to detect stimulation of erythropoiesis: K. Ramirez Cuevas, et al.; Drug Test. Anal. (Epub ahead of print) (2019)