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AdipoGen Life Sciences
Ac-Pro-Ala-Leu-AMC
65
CHF
CHF 65.00
In stock
AG-CP3-0036-M0011 mgCHF 65.00
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| Product Details | |
|---|---|
| Synonyms | Ac-PAL-AMC; Immunoproteasome Substrate |
| Product Type | Chemical |
| Properties | |
| Formula |
C26H34N4O6 |
| MW | 498.6 |
| Sequence |
Acetyl-Pro-Ala-Leu-7-amido-4-methylcoumarin |
| CAS | 1431362-79-6 |
| Purity Chemicals | ≥97% |
| Appearance | Lyophilized powder. |
| Solubility | Soluble in DMSO. |
| Other Product Data |
Use: After preparing a stock solution in DMSO (≥10mM) store product at -20°C to -80°C. It is recommended to make multiple aliquots after the first thaw to ensure best performance. Caspase-like activity can be measured using a typical working concentration range from 10-50µM. |
| InChi Key | BWJPVHDZSJFFDM-NDXORKPFSA-N |
| Smiles | O=C(C)N1CCC[C@H]1C(N[C@@H](C)C(N[C@@H](CC(C)C)C(NC2 =CC=C(C(C)=CC(O3)=O)C3=C2)=O)=O)=O |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
Avoid freeze/thaw cycles. Protect from light. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
- Ac-PAL-AMC is a fluorogenic peptide substrate for measuring caspase-like activity of the immunoproteasome.
- Hydrolysis of this substrate by the β1i/LMP2-subunit of the immunoproteasome is monitored by observing fluorescence at an Excitation wavelength of 345nm and Emission at 445nm.
- This substrate is specific to the immunoproteasome and is not hydrolyzed efficiently by the constitutive proteasome.
Product References
- Characterization of a new series of non-covalent proteasome inhibitors with exquisite potency and selectivity for the 20S β5-subunit: C. Blackburn, et al.; Biochem. J. 430, 461 (2010)
- PSMB9 codon 60 polymorphisms have no impact on the activity of the immunoproteasome catalytic subunit B1i expressed in multiple types of solid cancer: J.E. Park, et al.; PloS one 8, e73732 (2013)
- Inhibitors of the immunoproteasome: current status and future directions: Z. Miller, et al.; Curr. Pharm. Des. 19, 4140 (2013)
- Development and characterization of selective immunoproteasome inhibitors: C. Dubiella; Diss. Universitaet Muenchen (2015)
- Cleavage specificity of Mycobacterium tuberculosis ClpP1P2 protease and identification of novel peptide substrates and boronate inhibitors with anti-bacterial activity: T. Akopian, et al.; J. Biol. Chem. 290, 11008 (2015)
- Discovery of highly selective inhibitors of the immunoproteasome low molecular mass polypeptide 2 (LMP2) subunit: H.W.B. Johnson, et al.; ACS Med. Chem. Lett. 8, 413 (2017)
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