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AdipoGen Life Sciences
Ac-Ala-Asn-Trp-AMC
65
CHF
CHF 65.00
In stock
AG-CP3-0037-M0011 mgCHF 65.00
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| Product Details | |
|---|---|
| Synonyms | Ac-ANW-AMC; Immunoproteasome Substrate |
| Product Type | Chemical |
| Properties | |
| Formula |
C30H32N6O7 |
| MW | 588.6 |
| Sequence |
Acetyl-Ala-Asn-Trp-7-amido-4-methylcoumarin |
| Purity Chemicals | ≥97% |
| Appearance | Lyophilized powder. |
| Solubility | Soluble in DMSO. |
| Identity | Determined by MS. |
| Other Product Data |
Peptide Content: ≥94% Use: After preparing a stock solution in DMSO (≥10mM) store product at -20°C to -80°C. It is recommended to make multiple aliquots after the first thaw to ensure best performance. Chymotrypsin-like activity can be measured using a typical working concentration range from 10-50µM. |
| InChi Key | ORTKHFLJSJQDKO-ZCWWJEROSA-N |
| Smiles | O=C([C@@H](NC(C)=O)C)N[C@@H](CC(N)=O)C(N[C@@H](CC1=CNC2=C1C=CC=C2) C(NC3=CC=C(C(C)=CC(O4)=O)C4=C3)=O)=O |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
Avoid freeze/thaw cycles. Protect from light. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
- Ac-ANW-AMC is a fluorogenic peptide substrate for measuring chymotrypsin-like activity of the immunoproteasome.
- Hydrolysis of this substrate by the β5i-subunit of the immunoproteasome is monitored by observing fluorescence at an Excitation wavelength of 345nm and Emission at 445nm.
- This substrate is specific to the immunoproteasome and is not hydrolyzed efficiently by the constitutive proteasome.
Product References
- Elucidating the catalytic subunit composition of distinct proteasome subtypes: A crosslinking approach employing bifunctional activity-based probes: C.K. Cornish, et al.; ChemBioChem 16, 284 (2015)
- Pharmacodynamic monitoring of (immuno) proteasome inhibition during bortezomib treatment of a critically ill patient with lupus nephritis and myocarditis: K.A. De Groot, et al.; Lupus Sci. Med. 2, e000121 (2015)
- Immunoproteasome β5i-selective dipeptidomimetic inhibitors: P.K. Singh, et al.; ChemMedChem 11, 2127 (2016)
- Acetaldehyde suppresses the display of HBV-MHC class I complexes on HBV-expressing hepatocytes: M. Ganesan, et al.; Am. J. Physiol. Gastrointest. Liver Physiol. 317, G127 (2019)
- Monitoring the immunoproteasome in live cells using an activity-based peptide–peptoid hybrid probe: B.L. Zerfas & D.J. Trader; J. Am. Chem. Soc. 141, 5252 (2019)
- Evaluation of Immunoproteasome-Specific Proteolytic Activity Using Fluorogenic Peptide Substrates: S. Kim, et al.; Immune Netw. 22, e23 (2022)
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