CD40 – CD40L Pathway


CD40 and CD40L PathwayCD40 is a member of the TNF receptor family expressed by antigen-presenting cells (APCs) and B cells whereas its ligand, CD40L (CD154), is expressed by activated T cells. Interaction between CD40-CD40L stimulates cytokines secretion of B cells with subsequent T cell activation and antitumor immunity. This T cell priming effect of the CD40-CD40L pathway might be a useful approach in anticancer immunotherapy.


LIT: Cancer immunotherapy: activating innate and adaptive immunity through CD40 agonists: G.L. Beatty, et al.; Expert Rev. Anticancer Ther. 17, 175 (2017) • Multiple effects of CD40-CD40L axis in immunity against infection and cancer: A. Ara, et al.; Immunotargets Ther. 7, 55 (2018)





B Cell Expansion:  Highly Potent B Cell Activators and T Cell Priming Reagents

CD40 activation tools can be used to expand B cells, which, as antigen-presenting cells (APCs), are as effective as dendritic cells and promises to streamline the generation of antitumor CD8+ T cells. Several studies show that usage of the agonistic anti-CD40 antibody (FGK45) (Prod. No. AG-20B-0036PF) and MultimericCD40L (Prod. No. AG-40B-0010) are strong stimulators of antitumor immunity.

LIT: R.S. Kornbluth, et al.; Int. Rev. Immunol. 31, 279 (2012) • K.T. Byrne & R.H. Vonderheide; Cell Rep. 15, 2719 (2016)


POTENT  CD40L (human) (multimeric) (rec.)  (Prod. No. AG-40B-0010)

Figure: CD40L (human) (multimeric) (rec.) (Prod. No. AG-40B-0010) does not need an enhancer to induce B cell activation.




   CD40L (human) (multimeric) (rec.)

POTENT  anti-CD40 (mouse), mAb (FGK45) (Prod. No. AG-20B-0036) 

Figure: Systemic immune activation by CD40 ligation. Mice were sacrificed on day 8 after daily treatment on day 4-7 with FGK45 or control. FGK45 treatment elevated splenocyte numbers. *P < 0.005. Data represent mean ± SD for three to four mice per group.




   anti-CD40 (mouse), mAb (FGK45)


Biologically Active CD40 and CD40L Proteins  

Product Name PID Source Endotoxin Species
CD40 (human):Fc (human) (rec.) AG-40B-0083 HEK 293 cells <0.01EU/µg Human, Mouse
CD40 (human):Fc (human) (rec.) CHI-HF-210CD40 CHO cells <0.06EU/µg Human
CD40 (human)-muIg Fusion Protein

ANC-504-020

All Labeled Variants

CHO cells n.d. Human
CD40L (mouse) (multimeric) (rec.) AG-40B-0020 CHO cells <0.01EU/µg Human, Mouse
CD40L (human) (multimeric) (rec.)

AG-40B-0010

All Labeled Variants

CHO cells <0.01EU/µg Human
CD40L (human):Fc (human) (rec.) CHI-HF-210CD40L CHO cells <0.06EU/µg Human
CD40L (human)-muCD8 Fusion Protein

ANC-505-020

All Labeled Variants

CHO cells n.d. Human
CD40L (rat) (multimeric) (rec.) AG-40B-0107 CHO cells <0.02EU/µg Human, Mouse, Rat

VALIDATED Antibodies for CD40 and CD40L Research

 

Product Name PID Isotype Applications Species
CD40 (mouse), mAb (FGK45) (PF) AG-20B-0036PF Rat IgG2a FACS, FUNC Mouse
CD40 (human), mAb (BE-1)

ANC-189-020

All Labeled Variants

Mouse IgG1 FACS, FUNC, IP Human
CD40 (human), mAb (EA-5)

ANC-300-020

All Labeled Variants

Mouse IgG1 FACS, FUNC Human, Rat
CD40L (human), mAb (rec.) (blocking) (hu5c8) AG-27B-6002PF Human IgG1κ FUNC, WB Human, Dog
CD40L (human), mAb (24-31)

ANC-353-020

All Labeled Variants

Mouse IgG1 FACS, FUNC, IHC, WB Human, Primate


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