PD-1 [CD279]/PD-L1 [CD274] Pathway

PD-1-PD-L1 PathwayPD-1 (Programmed cell death protein 1; CD279) is a type I transmembrane protein belonging to the CD28/CTLA-4 family of immune receptors. PD-L1 (CD274; B7-H1) and PD-L2 (B7-DC; CD273) are immuno-coinhibitory ligands of the B7 family binding to PD-1. The PD-1/PD-L1 or PD-L2 signaling pathway is a negative regulatory mechanism that inhibits T cell proliferation and cytokine production. Blockade of the PD-1/PD-L1 interaction enhances antitumor immunity. The PD-1 pathway plays a major role in the inhibition of self-reactive T cells and protection against autoimmune diseases. PD-1 and PD-L1 also exist as soluble forms. Elevated levels of soluble PD-1 (sPD-1) are shown in rheumatoid arthritis, skin sclerosis and autoimmune hepatitis. Levels of sPD-L1 are increased in the plasma of cancer patients as well as in cerebrospinal fluid of gliomas. sPD-L1 is a biomarker of poor survival in patients with B cell lymphoma, renal cell carcinoma, metastatic melanoma or lung cancer and is associated with advanced tumor stages. 

LIT:  PD-1/PD-L1 blockade in cancer treatment: perspectives and issues: J. Hamanishi, et al.; Int. J. Clin. Oncol. 21, 462 (2016) • PD-1/PD-L1 immune checkpoint: Potential target for cancer therapy: F.K. Dermani, et al.; J. Cell Physiol. 234, 1313 (2019)

NEW: PD-1 (human) ELISA Kit  - Biomarker for Immuno-Oncology & Autoimmune Diseases

The PD-1 (human) ELISA Kit (Prod. No. AG-45B-0015) is a sandwich ELISA for specific quantitative determination of human soluble PD-1 in serum, plasma and cell culture supernatants. This ELISA Kit shows a very high specificity and sensitivity (1.6pg/ml).

Download the ELISA Kit Manual

NEW: PD-L1 (human) ELISA Kit  - Tumor Biomarker for Poor Survival Prognosis

The PD-L1 (human) ELISA Kit (Prod. No. AG-45B-0016) is a sandwich ELISA for specific quantitative determination of human soluble PD-L1 in serum, plasma and cell culture supernatants. This ELISA Kit shows a very high specificity and sensitivity (0.8pg/ml).

Download the ELISA Kit Manual

PD-L1 (mouse):Fc (mouse) (rec.)  - Biologically Active Proteins

PD-L1 (mouse):Fc (mouse) (rec.) (Prod. No. CHI-MF-110PDL1) is a biologically active recombinant fusion protein that inhibits anti-CD3/CD28 antibody-induced proliferation of mouse T lymphocytes.

Biological Activity: T cells from C57BL/6 mice were activated with coated anti-CD3/CD28 and PD-L1-Fc or control antibody (10µg/ml). Plots illustrate the percentage of dividing cells by using anti-CD44 and the dye Cell Trace Violet (CTV).
   PD-L1 (mouse):Fc (mouse) (rec.)

UNIQUE  anti-PD-1 (mouse), mAb (blocking) (1H10) 

AdipoGen Life Sciences' anti-PD-1 (mouse), mAb (blocking) (1H10) (Prod. No. AG-20B-0075) is a monoclonal antibody that recognizes mouse PD-1 and works specifically in Flow Cytometry and Functional Application. The antibody blocks PD-1 binding and induces a rapid activation and proliferation of T cells at concentration of 0.25µg/2x105 cells. The antibody is available with or without preservatives.

Figure: PD-1 receptor-induced CD4 T cell activation and proliferation by PD-1 (mouse), mAb (blocking) (1H10) (AG-20B-0075).

Magnetic bead affinity purified CD4+ T cells from C57BL/6 mice are stimulated in vitro with PD-1 (mouse), mAb (blocking) (1H10), anti-CD28 and rat IgG2a isotype (control Ig) (0.25µg/2x105 cells) for 48h. Proliferation is determined by [3H]thymidine incorporation. The presence of anti-CD28 mAb increases 1H10 mAb-mediated proliferation.

   anti-PD-1 (mouse), mAb (blocking) (1H10)

NEW IHC-Competent Antibodies for PD-1 and PD-L1 Staining

AdipoGen Life Sciences' anti-PD-1 (human), mAb (AG-IHC001) (Prod. No. AG-20B-6020) and anti-PD-L1 (human), mAb (AG-IHC411) (Prod. No. AG-20B-6022) are monoclonal antibodies that recognize human PD-1 and PD-L1, respectively, and work specifically in Immunohistochemistry Application. The antibodies are available in catalog and BULK sizes.

Figure: IHC Staining of PD-1 in human tonsil tissue using anti-PD-1 (human), mAb (AG-IHC001) (Prod. No. AG-20B-6020) (left side).

Figure: IHC Staining of PD-L1 in human lung tissue using anti-PD-L1 (human), mAb (AG-IHC411) (Prod. No. AG-20B-6022) (right side).

  anti-PD-L1 (human), mAb (AG-IHC411);anti-PD-1 (human), mAb (AG-IHC001)

Biologically Active PD-1 and PD-L1 Proteins  

Product Name PID Source Endotoxin Species
PD-1 (mouse):Fc (mouse) (rec.) CHI-MF-110PD1 CHO cells <0.06EU/µg Mouse
PD-1 (mouse):Fc (human) (rec.) CHI-MF-111PD1 HEK 293 cells <0.06EU/µg Mouse
PD-1 (human) (rec.) (untagged) CHI-HF-200PD1 HEK 293 cells <0.01EU/µg Human
PD-1 (human) (rec.) (His) CHI-HF-201PD1 HEK 293 cells <0.01EU/µg Human
PD-1 (human):Fc (human) (rec.) CHI-HF-210PD1 CHO cells <0.06EU/µg Human
PD-1 (human):Fc (human) (rec.) (non-lytic) CHI-HF-220PD1 CHO cells <0.06EU/µg Human
PD-1 (human):Fc (mouse) (rec.) CHI-HF-211PD1 HEK 293 cells <0.005EU/µg Human
PD-L1 (mouse):Fc (mouse) (rec.) (non-lytic) CHI-MF-120PDL1 CHO cells <0.06EU/µg Mouse
PD-L1 (human) (rec.) (untagged) CHI-HF-200PDL1 HEK 293 cells <0.01EU/µg Human
PD-L1 (human) (rec.) (His) CHI-HF-201PDL1 HEK 293 cells <0.01EU/µg Human
PD-L1 (human):Fc (human) (rec.) CHI-HF-210PDL1 CHO cells <0.06EU/µg Human
PD-L1 (human):Fc (human) (rec.) (non-lytic) CHI-HF-220PDL1 CHO cells <0.06EU/µg Human
PD-L1 (human):Fc (mouse) (rec.) CHI-HF-211PDL1 HEK 293 cells <0.005EU/µg Human
PD-L2 (mouse):Fc (mouse) (rec.) CHI-MF-110PDL2 CHO cells <0.06EU/µg Mouse
PD-L2 (human):Fc (human) (rec.) CHI-HF-210PDL2 CHO cells <0.06EU/µg Human
PD-L2 (human):Fc (human) (rec.) (non-lytic) CHI-HF-220PDL2 CHO cells <0.06EU/µg Human
PD-L2 (human):Fc (mouse) (rec.) CHI-HF-211PDL2 CHO cells <0.06EU/µg Human
Other PD-1 and PD-L1 Proteins

VALIDATED Antibodies for PD-1 and PD-L1 Research


Product Name PID Isotype Applications Species
PD-1 (mouse), mAb (blocking) (1H10) (PF) AG-20B-0075PF Rat IgG2aκ FACS, FUNC Mouse
PD-1 (human), mAb (ANC4H6)


All Labeled Variants

Mouse IgG1κ FACS Human
PD-1 (human), mAb (AG-IHC001) AG-20B-6020 Mouse IgG1κ IHC, Human
PD-L1 (human), mAb (ANC6H1)


All Labeled Variants

Mouse IgG1κ FACS Human
PD-L1 (human), mAb (AG-IHC411) AG-20B-6022 Mouse IgG1κ IHC, Human
PD-L2 (human), mAb (ANC8D12)


All Labeled Variants

Mouse IgG2aκ FACS, FUNC Human

Select Your Pathway of Interest  & Discover High Quality Reagents

© 2017 Adipogen Life Sciences. Pictures: © 2012 Martin Oeggerli. All Rights Reserved.