OX40 – OX40L Pathway
OX40 (CD134; TNFRSF4) is an activating receptor expressed on the surface of activated cytotoxic T cells and regulatory T cells (Tregs). OX40 plays a dual role in the immune response, both activating and amplifying T cell responses. On cytotoxic T cells, OX40 binds to its ligand OX40L (CD252; TNFSF4), resulting in stimulatory signals that promote T cell reproduction, function and survival. OX40/OX40L signaling blocks the ability of Tregs to suppress T cells and reduces Treg generation. By inhibiting the immunosuppressive effect of Tregs and limiting their population, OX40 further amplifies the impact of T cell activation. The dual effects of OX40 help to create a tumor microenvironment that is more favorable to the antitumor immune response.
LIT: OX40: Structure and function - What questions remain? J. Willoughby, et al.; Mol. Immunol. 83, 13 (2017) • The immunobiology of CD27 and OX40 and their potential as targets for cancer immunotherapy: S.L. Buchan, et al.; Blood 131, 39 (2018)
Biologically Active OX40 and OX40L Proteins
| Product Name | PID | Source | Endotoxin | Species |
| OX40 (mouse):Fc (human) (rec.) | CHI-MF-111CD134 | HEK 293 cells | <0.005EU/µg | Mouse |
| OX40 (human) (rec.) (His) | CHI-HR-200CD134 | E. coli | <0.1EU/µg | Human |
| OX40 (human):Fc (human) (rec.) | AG-40B-0014 | HEK 293 cells | <0.01EU/µg | Human, Mouse |
| OX40 (human):Fc (human) (rec.) | CHI-HF-210CD134 | CHO cells | <0.06EU/µg | Human |
| OX40 (human):Fc (mouse) (rec.) | CHI-HF-211CD134 | HEK 293 cells | <0.005EU/µg | Human |
| CD134 [OX40] (human)-muIg Fusion Protein |
ANC-513-020 |
CHO cells | n.d. | Human |
| OX40L (mouse) (multimeric) (rec.) | AG-40B-0029 | HEK 293 cells | <0.01EU/µg | Human, Mouse |
| Fc (human):OX40L, Soluble (human) (rec.) | AG-40B-0172 | HEK 293 cells | <0.01EU/µg | Human |
| OX40L (human) (rec.) (His) | CHI-HF-201CD252 | HEK 293 cells | <0.01EU/µg | Human, Mouse |
| OX40L (human):Fc (mouse) (rec.) | CHI-HF-211CD252 | HEK 293 cells | <0.005EU/µg | Human, Mouse |
| CD252 [OX40L] (human)-muCD8 Fusion Protein |
ANC-512-020 |
CHO cells | n.d. | Human |
VALIDATED Antibodies for OX40 and OX40L Research
| Product Name | PID | Isotype | Applications | Species |
| OX40 (human), mAb (BerAct35) |
ANC-355-020 |
Mouse IgG1 | FACS, IHC, WB | Human |
| OX40 (human), Rabbit Monoclonal (RM313) | REV-31-1199-00 | Rabbit IgG1 | IHC, WB | Human |
| OX40L (human), mAb (rec.) (blocking) (R4930) (PF) | AG-27B-6001PF | Human IgG1κ | FACS, FUNC | Human |
| OX40L (human), mAb (ANC10G1) |
ANC-400-020 |
Mouse IgG1κ | FACS, FUNC | Human |